We in the present study observed the effect of N-fructose modified chitosan quaternary ammonium derivativeson on rat skin wound healing through animal experiments. Forty rats were randomly divided into eight groups (5 in each group). Four groups among the all 8 groups were the experimental groups, while the other 4 groups were the control groups. Next to the skin along the back of the spine, 1.50 cm×2.00 cm×0.16 cm full-thickness skin was cut to make an excision wound model for every rat. Those in the experimental groups were treated with the N-fructose-modified chitosan quaternary ammonium derivatives ointment dressing the wound, while those in the control groups with sterile medical vaseline processing. We dressed the wounds twice a day to observe the wound healing of all rats in different groups. We then observed the wound healing and wound pathology after 3, 7, 10, 15 days respectively in different groups. Results showed significant differences of the time of wound healing, area of wound healing and volume of wound healing between the experimental groups and control groups (P<0.05). It can be well concluded that N-fructose-modified chitosan quaternary ammonium derivatives does not harm the skin, but could promote skin healing, so that they could be suitable skin repair materials and ideal raw materials for medical dressing.
Citation：
YUZhen, ZHANGZhiliang, YINHongping, XIONGWenshuo. Promotion of Rat Skin Healing by Using Natural Polymer Chitosan Derivatives. Journal of Biomedical Engineering, 2014, 31(1): 142-145. doi: 10.7507/1001-5515.20140028
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- 2. CHEN M C, WONG H S, LIN K J, et al. The characteristics, biodistribution and bioavailability of a chitosan-based nanoparticulate system for the oral delivery of heparin[J]. Biomaterials, 2009, 30(34):6629-6637.
- 3. TCHEMTCHOUA V T, ATANASOVA G, AQIL A, et al. Development of a procedure to simultaneously isolate RNA, DNA, and proteins from characterizing cells invading or cultured on chitosan scaffolds[J]. Anal Biochem, 2009, 393(1):145-147.
- 4. VAN TOAN N, NG C H, AYE K N, et al. Production of high-quality chitin and chitosan from preconditioned shrimp shells[J]. J Chem Tech Biotech, 2006, 81(7):1113-1118.
- 5. MORI T, MURAKAMI M, OKUMURA M, et al. Mechanism of macrophage activation by chitin derivatives[J]. J Vet Med Sci, 2005, 67(1):51-56.
- 6. LAI H L, ABU'KHALIL A, CRAIG D Q. The preparation and characterisation of drug-loaded alginate and chitosan sponges[J]. Int J Pharm, 2003, 251(1-2):175-181.
- 7. 熊文说,孙洋,张杨,等.水溶性壳聚糖的制备及其抗氧化活性的研究[C]//2010年中国药学大会暨第十届中国药师周论文集.天津:2010.
- 8. SUGUNA L, SINGH S, SIVAKUMAR P, et al. Influence of Terminalia chebula on dermal wound healing in rats[J]. Phytother Res, 2002, 16(3):227-231.
