EFFECT OF CURCUMIN ON CALCITIONIN GENE RELATED PEPTIDE EXPRESSION AFTER SPINAL CORD INJURY IN RATS_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

SUN Danhua , XU Jiwei.

Faculty of Basic Medicine, Luohe Medical College, Luohe Henan, 462002, P.R.China. Corresponding author: XU Jiwei, E-mail: xjwabc@163.com;

Corresponding author：

Keywords：

Curcumin; Spinal cord iniury; Calcitionin gene related peptide; Rat

DOI：

10.7507/1002-1892.20130268

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To investigate the effect of curcumin on calcitionin gene related peptide (CGRP) expression after spinal cord injury (SCI) in rats. Methods A total of 200 rats, weighing 250-300 g, were randomly divided into 4 groups (n=50): sham-operation group, normal saline (NS) group, low-dose curcumin group (30 mg/kg), and high-dose curcumin group (100 mg/kg). In sham-operation group, only vertebral lamina excision was performed without SCI; the SCI model was established in the other 3 groups. At immediate after modeling, 30 mg/kg and 100 mg/kg curcumin were injected intraperitoneally in 2 curcumin groups, equivalent NS was given in NS group (30 mg/kg), but no treatment in sham-operation group. At 1, 3, 7, 14, and 21 days after operation, the motor neural function was evaluated by the inclined plane test and Basso-Beattie-Bresnahan (BBB) scores; immunohistochemical staining and Western blot assay were used to observe CGRP expression. Results BBB score and inclined plane test score of NS group, low-dose curcumin group, and high-dose curcumin group were significantly lower than those of sham-operation group at each time point (P lt; 0.05). BBB score of low-dose curcumin group and high-dose curcumin group was significantly higher than that of NS group at 3, 7, 14, and 21 days after SCI (P lt; 0.05), and the score of high-dose group was significantly higher than that of low-dose curcumin group at 7, 14, and 21 days after SCI (P lt; 0.05). Inclined plane test score of low-dose curcumin group and high-dose curcumin group was significantly higher than that of NS group at 7, 14, and 21 days after SCI (P lt; 0.05), and the score of high-dose curcumin group was significantly higher than that of low-dose curcumin group at 7, 14, and 21 days after SCI (P lt; 0.05). Immunohistochemical staining results showed that the CGRP positive cells of sham-operation group was significantly more than those of the other 3 groups, and the CGRP positive cells of high-dose curcumin group were significantly more than those of low-dose curcumin group at each time point (P lt; 0.05); the CGRP positive cells of low- and high-dose curcumin groups were significantly more than those of NS group at 3, 7, 14, and 21 days after SCI (P lt; 0.05). Western blot assay results showed that the CGRP protein expressed at each time point after SCI in sham-operation group; the CGRP protein expression gradually decrease with time passing in NS group; but the CGRP protein expression gradually increased with time passing in low- and high-dose curcumin groups, and reached the peak at 14 days, then maintained a high level. Conclusion After SCI in rats, 30 mg/kg curcumin can improve rats’ motor function, and 100 mg/kg curcumin effect is more obvious, especially in promoting the expression of CGRP. That may be the mechanism of protection of the nervous system.

Citation： SUN Danhua,XU Jiwei.. EFFECT OF CURCUMIN ON CALCITIONIN GENE RELATED PEPTIDE EXPRESSION AFTER SPINAL CORD INJURY IN RATS. Chinese Journal of Reparative and Reconstructive Surgery, 2013, 27(10): 1225-1229. doi: 10.7507/1002-1892.20130268 Copy

1.	Aggarwal BB, Harikumar KB. Potential therapeutic effects of curcumin, the anti-inflammatory agent, against neurodegenerative, cardiovascular, pulmonary, metabolic, autoimmune and neop lastic diseases. Int J Biochem Cell Biol, 2009, 41(1): 40-59.
2.	Begum AN, Jones MR, Lim GP, et al. Curcumin structure-function, bioavailability, and efficacy in models of neuroinflammation and Alzheimer’s disease. J Pharmacol Exp Ther, 2008, 326(1): 196-208.
3.	李高文, 徐英, 库宝善, 等. 姜黄素的中枢药理作用研究进展. 神经药理学报, 2011, 1(2): 48-58.
4.	章菲菲, 吕有灵, 童菊芳, 等. 降钙素基因相关肽刺激体外培养脊髓神经元细胞c-fos的表达. 胃肠病学, 2006, 11(5): 286-289.
5.	胡魁. 急性脊髓损伤大鼠自主神经异常反射与CGRP关系的研究. 济南: 山东大学, 2007.
6.	徐纪伟, 孙丹华, 刘喜民, 等. 硫酸软骨素酶ABC对大鼠脊髓损伤后生长相关蛋白43和神经胶质纤维酸性蛋白的影响. 中国修复重建外科杂志, 2010, 24(10): 1212-1216.
7.	邹明明, 薛兴森, 朱海涛, 等. 姜黄素促进大鼠脊髓损伤恢复的病理及行为学研究. 第三军医大学学报, 2011, 33(8): 765-770.
8.	张月娥. 硫辛酸对大鼠脊髓继发性损伤的保护作用及其机制的探讨. 石家庄: 河北医科大学, 2011.
9.	徐纪伟, 韩曙, 凌树才. 脊髓横断损伤后再生基因蛋白的表达. 解剖学报, 2008, 39(4): 454-459.
10.	张晓娟. 姜黄提取物对SK-N-SH细胞谷氨酸损伤保护及Bcl-2、Bax基因和蛋白表达的影响. 石家庄: 河北医科大学, 2012.
11.	黄新武, 李华, 张红, 等. 姜黄素联合阿司匹林对血管性痴呆大鼠学习记忆能力的影响. 中药药理与临床, 2012, 28(1): 62-65.
12.	Yang JH, Zhang YQ, Guo Z. Endogenous CGRP protects retinal cells against stress induced apoptosis in rats. Neurosci Lett, 2011, 501(2): 83-85.
13.	袁耀欣, 王四平, 王亚利, 等. 姜黄提取物对血管性痴呆模型小鼠内皮素及降钙素基因相关肽的影响. 中国老年学杂志, 2008, 28(11): 1052-1054.
14.	王岩, 闫丽儒, 纪辉, 等. β阻滞剂对急性期实验性脑出血大鼠的神经保护作用. 中风与神经疾病杂志, 2010, 27(8): 679-682.
15.	郝宪恩, 王鑫国, 李楠, 等. 姜黄素对大鼠脑缺血再灌注损伤的保护作用. 中药药理与临床, 2004, 20(1): 7-9.
16.	张正洪, 杨龙珍, 席刚明, 等. 神经生长因子及降钙素基因相关肽对局灶性脑缺血再灌注大鼠脑环磷酸腺苷反应元件结合蛋白mRNA表达的影响. 解剖学杂志, 2008, 31(3): 384-386.
17.	Sink KS, Walker DL, Yang Y, et al. Calcitonin gene-related peptide in the bed nucleus of the stria terminalis produces an anxiety-like pattern of behavior and increases neural activation in anxiety-related structures. J Neurosci, 2011, 31(5): 1802-1810.
18.	张亚琴. 内源性CGRP在应激性视网膜细胞凋亡中的作用及感觉神经因素的影响. 太原: 山西医科大学, 2011.
19.	范寿成. 大鼠脊髓冷冻损伤后脊髓中GAP-43和CGRP的表达变化. 昆明: 昆明医学院, 2009.
20.	李建国, 孟壮志, 刘海英, 等. 降钙素基因相关肽在舌下神经切断后大鼠舌下神经核中表达的变化. 江苏医药, 2012, 38(12): 1399-1400.
21.	许愿忠, 王瑞, 张建伟, 等. 不同延迟时间后修复大鼠坐骨神经缺损对CGRP表达的影响. 中国临床解剖学杂志, 2005, 23(6): 648-651.
22.	张亮. 银杏叶提取物对大鼠坐骨神经损伤后脊髓前角降钙素基因相关肽表达的影响. 石家庄: 河北医科大学, 2011.
23.	祁小桐, 蒋电明, 朱凤臣, 等. 姜黄素对大鼠急性脊髓损伤的保护作用及机制的初步研究. 创伤外科杂志, 2013, 15(3): 250-254.
24.	Shin DH, Seo EY, Pang B, et al. Inhibition of Ca2+-release-activated Ca2+ channel (CRAC) and K+ channels by curcumin in Jurkat-T cells. J Pharmacol Sci, 2011, 115(2): 144-154.

1. Aggarwal BB, Harikumar KB. Potential therapeutic effects of curcumin, the anti-inflammatory agent, against neurodegenerative, cardiovascular, pulmonary, metabolic, autoimmune and neop lastic diseases. Int J Biochem Cell Biol, 2009, 41(1): 40-59.
2. Begum AN, Jones MR, Lim GP, et al. Curcumin structure-function, bioavailability, and efficacy in models of neuroinflammation and Alzheimer’s disease. J Pharmacol Exp Ther, 2008, 326(1): 196-208.
3. 李高文, 徐英, 库宝善, 等. 姜黄素的中枢药理作用研究进展. 神经药理学报, 2011, 1(2): 48-58.
4. 章菲菲, 吕有灵, 童菊芳, 等. 降钙素基因相关肽刺激体外培养脊髓神经元细胞c-fos的表达. 胃肠病学, 2006, 11(5): 286-289.
5. 胡魁. 急性脊髓损伤大鼠自主神经异常反射与CGRP关系的研究. 济南: 山东大学, 2007.
6. 徐纪伟, 孙丹华, 刘喜民, 等. 硫酸软骨素酶ABC对大鼠脊髓损伤后生长相关蛋白43和神经胶质纤维酸性蛋白的影响. 中国修复重建外科杂志, 2010, 24(10): 1212-1216.
7. 邹明明, 薛兴森, 朱海涛, 等. 姜黄素促进大鼠脊髓损伤恢复的病理及行为学研究. 第三军医大学学报, 2011, 33(8): 765-770.
8. 张月娥. 硫辛酸对大鼠脊髓继发性损伤的保护作用及其机制的探讨. 石家庄: 河北医科大学, 2011.
9. 徐纪伟, 韩曙, 凌树才. 脊髓横断损伤后再生基因蛋白的表达. 解剖学报, 2008, 39(4): 454-459.
10. 张晓娟. 姜黄提取物对SK-N-SH细胞谷氨酸损伤保护及Bcl-2、Bax基因和蛋白表达的影响. 石家庄: 河北医科大学, 2012.
11. 黄新武, 李华, 张红, 等. 姜黄素联合阿司匹林对血管性痴呆大鼠学习记忆能力的影响. 中药药理与临床, 2012, 28(1): 62-65.
12. Yang JH, Zhang YQ, Guo Z. Endogenous CGRP protects retinal cells against stress induced apoptosis in rats. Neurosci Lett, 2011, 501(2): 83-85.
13. 袁耀欣, 王四平, 王亚利, 等. 姜黄提取物对血管性痴呆模型小鼠内皮素及降钙素基因相关肽的影响. 中国老年学杂志, 2008, 28(11): 1052-1054.
14. 王岩, 闫丽儒, 纪辉, 等. β阻滞剂对急性期实验性脑出血大鼠的神经保护作用. 中风与神经疾病杂志, 2010, 27(8): 679-682.
15. 郝宪恩, 王鑫国, 李楠, 等. 姜黄素对大鼠脑缺血再灌注损伤的保护作用. 中药药理与临床, 2004, 20(1): 7-9.
16. 张正洪, 杨龙珍, 席刚明, 等. 神经生长因子及降钙素基因相关肽对局灶性脑缺血再灌注大鼠脑环磷酸腺苷反应元件结合蛋白mRNA表达的影响. 解剖学杂志, 2008, 31(3): 384-386.
17. Sink KS, Walker DL, Yang Y, et al. Calcitonin gene-related peptide in the bed nucleus of the stria terminalis produces an anxiety-like pattern of behavior and increases neural activation in anxiety-related structures. J Neurosci, 2011, 31(5): 1802-1810.
18. 张亚琴. 内源性CGRP在应激性视网膜细胞凋亡中的作用及感觉神经因素的影响. 太原: 山西医科大学, 2011.
19. 范寿成. 大鼠脊髓冷冻损伤后脊髓中GAP-43和CGRP的表达变化. 昆明: 昆明医学院, 2009.
20. 李建国, 孟壮志, 刘海英, 等. 降钙素基因相关肽在舌下神经切断后大鼠舌下神经核中表达的变化. 江苏医药, 2012, 38(12): 1399-1400.
21. 许愿忠, 王瑞, 张建伟, 等. 不同延迟时间后修复大鼠坐骨神经缺损对CGRP表达的影响. 中国临床解剖学杂志, 2005, 23(6): 648-651.
22. 张亮. 银杏叶提取物对大鼠坐骨神经损伤后脊髓前角降钙素基因相关肽表达的影响. 石家庄: 河北医科大学, 2011.
23. 祁小桐, 蒋电明, 朱凤臣, 等. 姜黄素对大鼠急性脊髓损伤的保护作用及机制的初步研究. 创伤外科杂志, 2013, 15(3): 250-254.
24. Shin DH, Seo EY, Pang B, et al. Inhibition of Ca2+-release-activated Ca2+ channel (CRAC) and K+ channels by curcumin in Jurkat-T cells. J Pharmacol Sci, 2011, 115(2): 144-154.

Chinese Journal of Reparative and Reconstructive Surgery

EFFECT OF CURCUMIN ON CALCITIONIN GENE RELATED PEPTIDE EXPRESSION AFTER SPINAL CORD INJURY IN RATS

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content