Carbamazepine Promotes Liver Regeneration Following Partial Hepatectomy in Rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 LUSi-cong ,  LUHai-ming , ZHANGQi-shun

Department of General Surgery Ⅱ, The Fourth Affiliated Hospital, Guangxi University of Medical Sciences, Liuzhou 545005, Guangxi Zhuang Autonomous Region, China;

Corresponding author：

LUHai-ming, Email: 35946677@qq.com

Keywords：

Carbamazepine; Hepatectomy; Rat; Liver regeneration

DOI：

10.7507/1007-9424.20150345

Video：

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Objective To explore the effect of carbamazepine (CBZ) on liver regeneration following partial hepatectomy (PH) in rats. Methods Sixty male Sprague-Dawley rats were randomly divided into CBZ group and control group, thirty rats in every group, all the rats were underwent 70% hepatectomy. In the CBZ group, all the rats were feed with 5% CBZ at a dose 250 mg/kg at 2 hours before PH, everyday after PH, and the last administration was at 2 hours before sacrifice. In the control group, all the rats were feed with the same quantitative and frequency dimethyl sulfoxide as 5% CBZ of the CBZ group. On day 1 before PH and on day 1, 2, 3, 5, and 8 after PH, the liver to body weight ratio, ALT, hepatocyte growth factor (HGF), and Ki-67 of each group were observed. Results ①On day 2, 3, 5 after PH, the liver to body weight ratios of the CBZ group were significantly higher than those of the control group (P < 0.05). On the other time point, there was no significant difference of the liver to body weight ratio between two groups (P > 0.05).②On all the time point, there was no significant difference of the serum ALT level between two groups (P > 0.05).③On day 1, 2, 3 after PH, the serum levels of HGF in the CBZ group were significantly higher than those in the control group (P < 0.05). On the other time point, there was no significant difference of the serum HGF level between two groups (P > 0.05).④On day 2 after PH, the expression of Ki-67 in the CBZ group was significantly higher than that in the control group (P < 0.05). On the other time point, there was no significant difference of the expression of Ki-67 between two groups (P > 0.05). Conclusion The results suggest that administration of CBZ has a promotive effect on liver regeneration following PH in rats and this effect might be through promotion of hepatocyte proliferation.

Citation： LUSi-cong, LUHai-ming, ZHANGQi-shun. Carbamazepine Promotes Liver Regeneration Following Partial Hepatectomy in Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2015, 22(11): 1319-1322. doi: 10.7507/1007-9424.20150345 Copy

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13.	Abdo EE, Cunha JE, Deluca P, et al. Protective effect of N2-mercaptopropionylglycine on rats and dogs liver during ischemia/reperfusion process. Arq Gastroenterol, 2004, 40(3):177-180.
14.	Zimmers TA, McKillop IH, Pierce RH, et al. Massive liver growth in mice induced by systemic interleukin 6 administration. Hepatology, 2003, 38(2):326-334.
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1. Balzan S, Belghiti J, Farges O, et al. The "50-50 criteria" on postoperative day 5:an accurate predictor of liver failure and death after hepatectomy. Ann Surg, 2005, 242(6):824-828.
2. Coyle JT, Duman RS. Finding the intracellular signaling pathways affected by mood disorder treatments. Neuron, 2003, 38(2):157-160.
3. Hidvegi T, Ewing M, Hale P, et al. An autophagy-enhancing drug promotes degradation of mutant alpha1-antitrypsin Z and reduces hepatic fibrosis. Science, 2010, 329(5988):229-232.
4. Mitchell C, Willenbring H. A reproducible and well-tolerated method for 2/3 partial hepatectomy in mice. Nat Protoc, 2008, 3(7):1167-1170.
5. Cataldegirmen G, Zeng S, Feirt N, et al. RAGE limits regeneration after massive liver injury by coordinated suppression of TNF-alpha and NF-kappaB. J Exp Med, 2005, 201(3):473-484.
6. 陈万青, 张思维, 郑荣寿, 等.中国2009年恶性肿瘤发病和死亡分析.中国肿瘤, 2013, 22(1):2-12.
7. Schindl MJ, Redhead DN, Fearon KC, et al. The value of residual liver volume as a predictor of hepatic dysfunction and infection after major liver resection. Gut, 2005, 54(2):289-296.
8. Mao S, Glorioso JM, Nyberg SL. Liver regeneration. Transl Res, 2014, 163(4):352-362.
9. 李斌, 刘辰, 易滨, 等.肝切除联合胆肠内引流对梗阻性黄疸大鼠的影响.中国普外基础与临床杂志, 2011, 18(12):1271-1277.
10. Michalopoulos GK, DeFrances MC. Liver regeneration. Science, 1997, 276(5309):60-66.
11. Andersen KJ, Knudsen AR, Kannerup AS, et al. The natural history of liver regeneration in rats:description of an animal model for liver regeneration studies. Int J Surg, 2013, 11(9):903-908.
12. 潘立超, 任为正, 葛新兰, 等.门静脉联合胆管双结扎与单纯门静脉结扎对非结扎侧肝增生的影响.中国现代普通外科进展, 2015, 18(4):253-258.
13. Abdo EE, Cunha JE, Deluca P, et al. Protective effect of N2-mercaptopropionylglycine on rats and dogs liver during ischemia/reperfusion process. Arq Gastroenterol, 2004, 40(3):177-180.
14. Zimmers TA, McKillop IH, Pierce RH, et al. Massive liver growth in mice induced by systemic interleukin 6 administration. Hepatology, 2003, 38(2):326-334.
15. 毛拉艾沙·买买提, 易超, 依马木买买提江·阿布拉, 等.肝再生增强因子对肝硬化大鼠50%肝切除术后余肝再生的影响.中华实验外科杂志, 2014, 31(8):1725-1728.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Carbamazepine Promotes Liver Regeneration Following Partial Hepatectomy in Rats

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