ObjectiveTo investigate the methodology of a newly developed highpressure liquid chromatography electrochemical system in urinary 8hydroxydeoxyguanosine (8OHdG) quantification. MethodsQuantification of urinary 8OHdG with ESA 5600 Coularray system among 21 children from liver cancer highrisk areas, Fusui country, in contrast with 63 controls from Nanning city, Guangxi province.ResultsThe resolution, sensitivity, linearity, precision and recovery of this approach all fully meet the requirement of routine urinary 8OHdG quantification. The mean 8OHdG level of this population was (5.26±0.41) ng/mg creatinine, higher than previous report 〔(4.62±0.091) ng/mg creatinine〕 by similar method.ConclusionWith cautious design and modification, this method is reliable and accurate in high throughput quantification of urinary 8OHdG.
Citation：
PENG Tao,YAN Lnan,LIU Zhiming,Hiroshi Kasai,PENG Minhao,LI Lequn,Kazuyoshi Tamae,QIN Xue,WEI Zhongliang,LIANG Renxiang,LI Jiaquan. Measurement of Urinary 8Hydroxydeoxyguanosine Level with a HighPressure Liquid Chromatography Eectrochemical Approach. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2003, 10(3): 281-283. doi:
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- 1. Halliwell B. Free radical, antioxidants, and human disease: curiosity, cause, or consequence? [J]. Lancet, 1994； 344(13)∶721.
- 2. Grollman AP, Moriya M. Mutagenesis by 8oxoguanine: an enemy within [J]. Trends Genet, 1993； 9(7)∶246.
- 3. Kasai H. Analysis of a form of oxidative DNA damage, 8hydroxy2’deoxyguanosine, as a marker of cellular oxidative stress during carcinogenesis [J]. Mutat Res, 1997； 387(3)∶147.
- 4. Kasai H, IwamotoTanaka N, Miyamoto T, et al. Life style and urinary 8hydroxydeoxyguanosine, a marker of oxidative dna damage: effects of exercise, working conditions, meat intake, body mass index, and smoking [J]. Jpn J Cancer Res, 2001； 92(1)∶9.
- 5. Bogdanov MB, Beal MF, McCabe DR, et al. A carbon columnbased liquid chromatography electrochemical approach to routine 8hydroxy2’deoxyguanosine measurements in urine and other biologic matrices: a oneyear evaluation of methods [J]. Free Radic Biol Med, 1999； 27(5-6)∶647.
- 6. Tagesson C, Chabiuk D, Axelson O,et al. Increased urinary excretion of the oxidative DNA adduct, 8hydroxydeoxyguanosine, as a possible early indicator of occupational cancer hazards in the asbestos, rubber, and azodye industies [J]. Pol J Occup Med Environ Health, 1993； 6(4)∶357.
- 7. Loft S, Vistisen K, Ewertz M, et al. Oxidative DNA damage estimated by 8hydroxydeoxyguanosine excretion in humans: influence of smoking, gender and body mass index [J]. Carcinogenesis, 1992； 13(12)∶2241.
- 8. Acworth IN, McCabe DR, Maher TJ. The analysis of free radical, their reaction products, and antioxidants. In: Baskin SI, Salem H. Eds. Oxidants, antioxidants, and free radicals [M]. Washington: Taylor & Francis， 1997∶23-77.
