【Abstract】Objective To investigate the injury of calcium on the liver. Methods By using collagenase-containing solution to perfuse the rat livers, the rat liver suspension with different viability was prepared, preserved hypothermically, and the cytosolic calcium comcentration was detected with Fura2/AM. Results ① The concentration of cytosolic calcium after 2-hour storage: Experiment group 1(viability 5%) (1055.0±30.79) nmol/L, experiment group 2(viability 10%) (853.0±20.42) nmol/L, experiment group 3(viability 30%) (616.0±13.20) nmol/L, experiment group 4(viability 50%) (562.0 ±26.06) nmol/L, experiment group 5(viability 70%-80%) (318.0±13.01) nmol/L, experiment group 6(viability 90%) (114.6±6.11) nmol/L. ②The concentration of cytosolic calcium after 24hour storage: Experiment group A(viability 10%) (1704.0±70.11) nmol/L, experiment group B(viability 50%) (1125.0±23.22) nmol/L. The results showed that the lower was the viability, the higher was the cytosolic calcium concentration.With the same viability the cytosolic calcium concentration elevated more than two times higher than the original concentration with the time lengthened. Conclusion Calcium overload is one of the main factors which attribute to the ischemiareperfusion injury of the hepatocytes.
Citation：
LIU Dongju,HE Sanguang.. THE INJURY OF CALCIUM OVERLOAD TO THE HEPATOCYTES IN COLD STORAGE. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2001, 8(6): 381-383. doi:
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- 1. Mora NP, Cienfuegos JA, Bernaldo de Quiros, et al. Successful liver allograft function after 24hour preservation: cumulative effects of prostacyclin plus verapamil〔Ｊ〕. Transplant Proceeding, 1987; 19(5)∶ 3932.
- 2. Ar Rajab A, Ahren B, Bengmark S. Improved liver preservation for transplantation due to calcium channel blockade 〔Ｊ〕. Transplantation, 1991; 51(5)∶ 965.
- 3. 刘东举， 刘永锋，赵日省等. 异搏定对大鼠肾脏低温保存时超微结构及移植肾功能的影响 〔Ｊ〕.中华器官移植杂志，1994； 15（2）∶ 57.
- 4. 卢绮萍，史陈让，吴笑春等.用Fura2 单细胞显微术动态观察肝缺血再灌注细胞内游离钙浓度 〔Ｊ〕.中华实验外科杂志，1993； 10（3）∶ 115.
- 5. Sakaguchi S, Tsutsumi E, Yokota K.Degense effects of a traditional chinese medicine (shosaikoto) against metablic disorders during endotoxemia: approached from the behavior of the calcium 〔Ｊ〕. Biological & Pharmaceutical Bulletin, 1994; 17(2)∶232.
- 6. 刘东举，崔宏，崔桂英等.钙通道阻滞剂对低温保存大鼠肝脏的保护作用 〔Ｊ〕. 中华器官移植杂志， 2001； 22(2)∶94.
- 7. Cui Guiying, Li jinming, Cui Hong,et al. Effects of calcium channel blockers on calcium releaseactivated calcium currents in rat hepatocytes 〔Ｊ〕. Acta Pharmacol Sin， 1999; 20(5)∶415.
