This prospective animal study was designed to investigate the changes of plasma endothelin (ET) levels in acute necrotizing pancreatitis (ANP). Sprague-Dawley rats were randomly devided into 3 groups: acute necrotizing pancreatitis (ANP) group in which ANP was induced by infusion of 5% sodium taurocholate (STC) into biliopancreatic duct, sham operation (SO) group and platelet activating factor antagonist BN50739 (BN) group. Blood levels of ET and platelet activating factor (PAF) were detected. Pancreatic microcirculatory blood flow was measured and pancreatic histological scores were evaluated. Results showed that the pancreatic microcirculatory blood flow in ANP group was decreased to a great extent immediatly after induction of ANP and soon began to rise slowly for 3 hours and again decreased steadily after that. The blood levels of ET, PAF and histological scores in ANP group were significantly higher than those in SO group. In BN group, the blood flow was significantly improved and the levels of blood ET, PAF and histological scores were all significantly lower as compared to those in ANP group. It is concluded that ischemia/ reperfusion is present in the initiation of acute necrotizing pancreatitis induced by STC in the rat. This leads to injuries of endothelial cells and increase in the production of ET and PAF. I/R lesions,and interaction of ET and PAF lead to a vicious circle, thus augmenting the pathological changes in the pancreas.
Citation：
Li Xi,Li Jieshou,Zhu Weiming,et al.. THE CHANGE AND SIGNIFICANCE OF PLASMA LEVELS OF ENDOTHELIN IN ACUTE NECROTIZING PANCREATITIS IN RATS. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 1999, 6(6): 331-333. doi:
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- 1. Takakuwa T, Endo S, Nakae H, et al. Relationships between plasma levels of typeI phospholipase A2, PAFacetylhydrolase, leukotriene B4, complements, endothelin1, and thrombomodulin in patients with sepsis. Res Commun Chem Pathol Pharmacol, 1994; 84(3)∶271.
- 2. Bassi D, Kollias N, FernandezDel Castillo C, et al. Impairment of pancreatic microcirculation correlates with the sererity of acute experimental pancreatitis. J Am Coll Surg, 1994; 179(3)∶257.
- 3. McMillen MA, Sumpio BE. Endothelins: polyfunctional cytokines. J Am Coll Surg, 1995; 180(5)∶621.
- 4. Aho HJ, Koskensalo SML, Nevalainen TJ. Experimental pancreatitis in the rat. Sodium taurocholateinduced acute haemorrhagic pancreatitis. Scand J Gastroenterol, 1980; 15(4)∶411.
- 5. Pinckard RN, Farr RS, Hanahan DJ. Physicochemical and functional identity of rabbit plateletactivating factor(PAF) released in vivo during IgE anaphylaxis with PAF released in vitro from IgE sensitized basophils. J Immunol, 1979; 123(4)∶1847.
- 6. Schmidt J, Lewandrowski K, FernanderDel Castillo C, et al. Histopathologic correlates of serum amylase activity in acute experimental pancreatitis. Dig Dis Sci, 1992; 37(9)∶1426.
- 7. Kusterer K, Poschmann T, Friedemann A, et al. Arterial constriction, ischemiareperfusion, and leukocyte adherence in acute pancreatitis. Am J Physiol, 1993; 265(1Pt1)∶G165.
- 8. Hoffmann TF, Leiderer R, Waldner H, et al. Ischemia reperfusion of the pancrease: a new in vivo model of acute pancreatitis in rats. Res Exp Med Berl, 1995; 195(3)∶125.
- 9. Anderson BO, Bensard DD, Harken AH. The role of platelet activating factor and its antagonists in shock, sepsis and multiple organ failure. Surg Gynecol Obstet, 1991; 1972(5)∶415.
- 10. Mustafa SB, Gandht CR, Harvey SAK, et al. Endothelin stimulates plateletactivating factor synthesis by cultured rat kupffer cells. Hepatology, 1995; 21(2)∶545.
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