目的 研究联合检测微卫星不稳定性( MSI) 和p16 基因启动子甲基化在早期肺癌中的诊断价值。方法 对肺癌、癌前病变和正常肺组织采用PCR 单链长度分析法检测MSI, 采用甲基化特异PCR 法检测p16 甲基化。结果 癌前病变组MSI 的发生率显著高于肺癌组( P lt;0. 05) 和正常肺组织组( P lt;0. 01) , 肺癌组MSI 的发生率显著高于正常肺组织组( P lt;0. 01) ; 肺癌组p16 甲基化的发生率显著高于癌前病变组( P lt;0. 01) 和正常肺组织组( P lt;0. 01) , 癌前病变组p16 甲基化的发生率显著高于正常肺组织组( P lt;0. 01) ; 联合检测MSI 和p16 甲基化的敏感性显著高于单一检测MSI( P lt;0. 01) 和p16 甲基化( P lt;0. 05) ; 联合检测法与单一检测MSI 和单一检测p16 甲基化的特异性比较,差异无显著性意义( P gt;0. 05) 。结论 联合检测MSI 和p16 甲基化可以显著提高早期肺癌诊断的敏感性同时不降低其特异性, 值得临床推广。
Citation：
廖秀清,赵麟丰,余时沧,郭武,王朝永. 联合检测微卫星不稳定性和p16甲基化在早期肺癌中的诊断价值. Chinese Journal of Respiratory and Critical Care Medicine, 2010, 9(1): 81-82. doi:
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- 1. 张鋆歆,申洪.肺增生病变FHIT基因微卫星不稳定和杂合性丢失的研究.第一军医大学学报,2003,23:313-317.
- 2. González-Quevedo R,García-Aranda C,Morán A,et al. Differential impact of p16 inactivation by promoter methylation in non-small cell lung and colorectal cancer:clinical implications. Int J Oncol,2004,24:349-355.
- 3. Kwong FM，Tang JC，Srivastava G，et a1. Inactivation mechanisms and growth suppressive effects of p16 INK4a in Asian esophageal squamous carcinoma cell lines[J]. Cancer Lett，2004，208: 207-213.
- 4. Dacic S,Lomago D,Hunt JL,et al. Microsatellite instability is uncommon in lymphoepithelioma-like carcinoma of the lung.Am J Clin Pathol,2007,127:282-286.
- 5. Melloni G,Doglioni C,Bandiera A,et al. Prognostic factors and analysis of microsatellite instability in resected pulmonary metastases from colorectal carcinoma. Ann Thorac Surg,Ann Thorac Surg,2006,81:2008-2013.
- 6. Hung J,Kishimoto Y,Sugio K,et al.Allele-specific chromosome 3p deletions occur at an early stage in the pathogenesis of lung carcinoma.JAMA,1995,273:558-563.
- 7. Malhotra P,Behera D,Srinivasan R,et a1.Detection of microsatellite alterations in bronchial washings in squamous cell lung cancer: the first study from India.Jpn J Clin Oncol,2004,34:439-444.
- 8. Bodner-Adler B,Bodner K,Czerwenka K,et al. Expression of p16 protein in patients with uterine smooth muscle tumors: an immunohistochemical analysis. Gynecol Oncol,2005,96:62-66.
- 9. Georgiou E,Valeri R,Tzimagiorgis G,et al.Aberrant p16 promoter methylation among Greek lung cancer patients and smokers: correlation with smoking. Eur J Cancer Prev,2007,16:396-402.
- 10. Palmisano WA,Divine KK,Saccomanno G,et al.Predicting lung cancer by detecting aberrant promoter methylation in sputum.Cancer Res,2000,60:5954-5958.
