目的 探讨HIF-1α和BAK蛋白在胃癌中的表达情况,以及二者在胃癌中的相互关系及作用。
方法 应用免疫组化SABC染色法检测80例胃癌组织和20例正常胃组织中的HIF-1α和BAK蛋白的表达情况。
结果 胃癌中HIF-lα和BAK蛋白的表达阳性率分别为56.3%(45/80)和67.5%(54/80),而在胃正常组织中分别为5.0%(1/20)和20.0%(4/20),二者在胃癌中的表达显著高于胃正常组织,其差异有统计学意义(P<0.05)。HIF-1α蛋白表达与胃癌组织的浸润范围、分化程度及淋巴结转移有关(P<0.05),与临床分期、年龄及性别无关(P>0.05);BAK蛋白表达与胃癌浸润及分化程度相关(P<0.05),与淋巴结转移、临床分期、年龄及性别无关(P>0.05)。胃癌组织中HIF-1α与BAK蛋白的阳性表达之间呈正相关(列联系数r=0.056,P<0.05)。
结论 HIF-1α与BAK蛋白在胃癌的临床分期及浸润转移中存在关系,这对于研究胃癌的发生和发展,以及对于探索以二者为靶点的抗肿瘤治疗有重要意义。
Citation:
张晓楠,荆宏生,史逸华,刘伟新,张英海. Expressions and Significances of HIF-1α and BAK in Gastric Cancer. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2012, 19(3): 322-324. doi:
Copy
Copyright © the editorial department of CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY of West China Medical Publisher. All rights reserved
1. |
Leslie H, Sobin L, Gospodarowicz M, et al. International Union Against Cancer (UICC) TNM classification of malignant tumours [M]. 7th edition. New York: Wiley-Liss, 2010:117-126.
|
2. |
陈振栋, 吉志武, 安树才, 等. HIF-1α 和MMP-2 在低分化胃癌中的表达及与浸润转移的关系 [J]. 中国普外基础与临床杂志, 2010, 17(2):187-188..
|
3. |
Zhong H, De Marzo AM, Laughner E, et al. Overexpression of hypoxia-inducible factor 1 alpha in common human cancers and their metastases [J]. Cancer Res, 1999, 59(22): 5830-5835.
|
4. |
宗建春.HIF-1 的生物学机制及在肿溜研究中的进展 [J]. 重庆医学, 2004, 33(5):780-782.
|
5. |
Subarsky P, Hill RP. The hypoxic tumour microenvironment and metastatic progression [J]. Clin Exp Metastasis, 2003, 20(3):237-250.
|
6. |
谷依学. 缺氧诱导因子1α 及血管内皮生长因子的表达与胃癌生物学行为的关系 [J]. 南华大学学报( 医学版), 2005, 33(1):13-16.
|
7. |
Semenza GL. Targeting HIF-1 for cancer therapy [J]. Nat Rev Cancer, 2003, 3(10): 721-732.
|
8. |
Birner P, Schindl M, Obermair A, et al. Overexpression of hypoxia-inducible factor 1 alpha is a marker for an unfavorable prognosis in early-stage invasive cervical cancer [J]. Cancer Res, 2000, 60(17): 4693-4696.
|
9. |
张欣然, 韩瑞珠, 等. bcl-2 家族促凋亡成员—— bak 与肿瘤[J]. 国外医学遗传学分册, 2005, 28(3):149-151.
|
10. |
Farrow SN, White JH, Martinou I, et al. Cloning of a bcl-2 homologue by interaction with adenovirus E1B 19K [J]. Nature,1995, 374(6524): 731-733.
|
11. |
Chittenden T, Harrington EA, O’connor R, et al. Induction of apoptosis by the Bcl-2 homologue Bak [J]. Nature, 1995,374(6524):733-736.
|
12. |
Chen G, Sordillo EM, Ramey WG, et al. Apoptosis in gastric epithelial cells is induced by Helicobacter pylori and accompanied by increased expression of BAK [J]. Biochem Biophys Res Common, 1997, 239(2): 626-632.
|
13. |
An WG, Kanekal M, Simon MC, et al. Stabilization of wildtype p53 by hypoxia inducible factor-1α [J]. Nature, 1998,392(6674): 405-408.
|
14. |
Volm M, Koomagi R. Hypoxia-inducible factor (HIF-1) and its relationship to apoptosis and proliferation in lung cancer [J].Anticancer Res, 2000, 20(3A): 1527-1533.
|
15. |
Jung F, Palmer LA, Zhou N, et al. Hypoxic regulation of inducible nitric oxide synthase via hypoxia inducible factor-1 in cardiac myocytes [J]. Circ Res, 2000, 86(3): 319-325.
|
16. |
Sasabe E, Tatemoto Y, Li D, et al. Mechanism of HIF-1 alphadependent suppression of hypoxia-induced apoptosis in squamous cell carcinoma cells [J]. Cancer Sci, 2005, 96(7): 394-402.
|
- 1. Leslie H, Sobin L, Gospodarowicz M, et al. International Union Against Cancer (UICC) TNM classification of malignant tumours [M]. 7th edition. New York: Wiley-Liss, 2010:117-126.
- 2. 陈振栋, 吉志武, 安树才, 等. HIF-1α 和MMP-2 在低分化胃癌中的表达及与浸润转移的关系 [J]. 中国普外基础与临床杂志, 2010, 17(2):187-188..
- 3. Zhong H, De Marzo AM, Laughner E, et al. Overexpression of hypoxia-inducible factor 1 alpha in common human cancers and their metastases [J]. Cancer Res, 1999, 59(22): 5830-5835.
- 4. 宗建春.HIF-1 的生物学机制及在肿溜研究中的进展 [J]. 重庆医学, 2004, 33(5):780-782.
- 5. Subarsky P, Hill RP. The hypoxic tumour microenvironment and metastatic progression [J]. Clin Exp Metastasis, 2003, 20(3):237-250.
- 6. 谷依学. 缺氧诱导因子1α 及血管内皮生长因子的表达与胃癌生物学行为的关系 [J]. 南华大学学报( 医学版), 2005, 33(1):13-16.
- 7. Semenza GL. Targeting HIF-1 for cancer therapy [J]. Nat Rev Cancer, 2003, 3(10): 721-732.
- 8. Birner P, Schindl M, Obermair A, et al. Overexpression of hypoxia-inducible factor 1 alpha is a marker for an unfavorable prognosis in early-stage invasive cervical cancer [J]. Cancer Res, 2000, 60(17): 4693-4696.
- 9. 张欣然, 韩瑞珠, 等. bcl-2 家族促凋亡成员—— bak 与肿瘤[J]. 国外医学遗传学分册, 2005, 28(3):149-151.
- 10. Farrow SN, White JH, Martinou I, et al. Cloning of a bcl-2 homologue by interaction with adenovirus E1B 19K [J]. Nature,1995, 374(6524): 731-733.
- 11. Chittenden T, Harrington EA, O’connor R, et al. Induction of apoptosis by the Bcl-2 homologue Bak [J]. Nature, 1995,374(6524):733-736.
- 12. Chen G, Sordillo EM, Ramey WG, et al. Apoptosis in gastric epithelial cells is induced by Helicobacter pylori and accompanied by increased expression of BAK [J]. Biochem Biophys Res Common, 1997, 239(2): 626-632.
- 13. An WG, Kanekal M, Simon MC, et al. Stabilization of wildtype p53 by hypoxia inducible factor-1α [J]. Nature, 1998,392(6674): 405-408.
- 14. Volm M, Koomagi R. Hypoxia-inducible factor (HIF-1) and its relationship to apoptosis and proliferation in lung cancer [J].Anticancer Res, 2000, 20(3A): 1527-1533.
- 15. Jung F, Palmer LA, Zhou N, et al. Hypoxic regulation of inducible nitric oxide synthase via hypoxia inducible factor-1 in cardiac myocytes [J]. Circ Res, 2000, 86(3): 319-325.
- 16. Sasabe E, Tatemoto Y, Li D, et al. Mechanism of HIF-1 alphadependent suppression of hypoxia-induced apoptosis in squamous cell carcinoma cells [J]. Cancer Sci, 2005, 96(7): 394-402.