Study on Relationship Between RasTG and Pancreatic Carcinoma_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

GONG Jun ¹ , XIANG Guangming ² , ZHENG Zhenjiang ² , TAN Chunlu ² , LIU Xubao ² , CHEN Yan. ³

1.Department of Hepatobiliary and Pancreatic Surgery, Sichuan Academy of Medical Science and Sichuan Provincial People’s Hospital, Chengdu 610072, Sichuan Province, China;;
2.Department of Hepatobiliary and Pancreatic Surgery, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China;;
3.Institute for Nutritional Sciences, SIBS, Chinese Academy of Sciences，Shanghai 200031，China;

Corresponding author：

Keywords：

Ras protein; RasTG gene; Pancreatic carcinoma

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

ObjectiveTo study the expressions of Ras trapping to Golgi (RasTG) genes in pancreatic carcinoma tissues and to observe the growth, proliferation and the impact of tumors formation of human pancreatic cancer cells (PANC-1), and to explore its mechanism. MethodsMade PANC1 as a target to research, transfected RasTG genes into PANC-1, used RNAi technology and observed the growth, proliferation and the impact of tumors formation of the cells. Meantime, contrasted the RasTG expressions between pancreatic ductal cancer and adjacent tissue by tissue microarray technology. Results①The expression of RasTG gene in tissues was not very differential， which was higher in the brain, liver, and adrenal gland. ②The expression of RasTG protein in pancreatic ductal carcinoma was significantly higher than that in adjacent tissues （P lt;0.05）. ③After RasTG RNAi in PANC-1 cells, the ability of growth and proliferation were decreased. ④The ability of tumors formation in PANC-1 cells after RNAi was decreased, carcinoma’s volume of transfected group was significantly smaller than that in the nontransfected group (P lt;0.05). ConclusionsRasTG gene is widely distributed in animals. RasTG protein in pancreatic carcinoma tissues is higher than that in adjacent tissues. The ability of proliferation, transformation and tumors formation in PANC-1 cells after RNAi of RasTG gene are restrained, RasTG gene is a positive regulatory factor.

Citation： GONG Jun,XIANG Guangming,ZHENG Zhenjiang,TAN Chunlu,LIU Xubao,CHEN Yan.. Study on Relationship Between RasTG and Pancreatic Carcinoma. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2011, 18(11): 1169-1174. doi: Copy

1.	Young A, Lyons J, Miller AL, et al. Ras signaling and therapies [J]. Adv Cancer Res, 2009, 102(09): 117.
2.	Shaw RJ, Cantley LC. Ras, PI(3)K and mTOR signalling controls tumour cell growth [J]. Nature, 2006, 441(7092): 424430.
3.	Tang YT, Hu T, Arterburn M, et al. PAQR proteins: a novel membrane receptor family defined by an ancient 7transmembrane pass motif [J]. J Mol Evol, 2005, 61(3): 372380.
4.	Zhu Y, Bond J, Thomas P. Identification, classification, and partial characterization of genes in humans and other vertebrates homologous to a fish membrane progestin receptor [J]. Proc Natl Acad Sci USA, 2003, 100(5): 22372242.
5.	Lin SG, Zhang R, Wang JQ. RNA interference technique and the using [J]. J Mod Lab Med, 2008, 23(4): 8386.
6.	Bivona TG, Pérez De Castro I, Ahearn IM, et al. Phospholipase cgamma activates Ras on the golgi apparatus by means of RasGRP1 [J]. Nature, 2003, 424(6949): 694698.
7.	Rehli M, Krause SW, Schwarzfischer L, et al. Molecular cloning of a novel macrophage maturationassociated transcript encoding a protein with several potential transmembrane domains [J]. Biochem Biophys Res Commun, 1995, 217(2): 661667.
8.	de Braud F, Cascinu S, Gatta G. Cancer of pancreas [J]. Crit Rev Oncol Hematol, 2004, 50(2): 147155.
9.	Mann O, Strate T, Schneider C, et al. Surgery for advanced and metastatic pancreatic cancer—current state and perspectives [J]. Anticancer Res, 2006, 26(1B): 681686.
10.	Riall TS, Nealon WH, Goodwin JS, et al. Pancreatic cancer in the general population: Improvements in survival over the last decade [J]. J Gastrointest Surg, 2006, 10(9): 12121223.
11.	Wenger FA, Peter F, Zieren J, et al. Prognosis factors in carcinoma of the head of the pancreas [J]. Dig Surg, 2000, 17(1): 2935.
12.	Wakeman CJ, Martin IG, Robertson RW, et al. Pancreatic cancer: management and survival [J]. ANZ J Surg, 2004, 74(11): 941944.
13.	Takhar AS, Palaniappan P, Dhingsa R, et al. Recent developments in diagnosis of pancreatic cancer [J]. BMJ, 2004, 329(7467): 668673.
14.	Sarkar FH, Banerjee S, Li Y. Pancreatic cancer: pathogenesis, prevention and treatment [J/OL]. Toxicol Appl Pharmacol, 2007, 224(3): 326336.
15.	Wright WE, Shay JW, Piatyszek MA. Modifications of a telomeric repeat amplification protocol (TRAP) result in increased reliability, linearity and sensitivity [J]. Nucleic Acids Res, 1995, 23(18): 37943795.
16.	Rozenblum E, Schutte M, Goggins M, et al. Tumorsuppressive pathways in pancreatic carcinoma [J]. Cancer Res, 1997, 57(9): 17311734.
17.	Chiu VK, Bivona T, Hach A, et al. Ras signalling on the endoplasmic reticulum and the Golgi [J]. Nat Cell Biol, 2002, 4(5): 343350.
18.	Hirai H, Okabe T, Anraku Y, et al. Activation of the cKras oncogene in a human pancreas carcinoma [J]. Biochem Biophys Res Commun, 1985, 127(1): 168174.
19.	Matsubayashi H, Watanabe H, Yamaguchi T, et al. Multiple Kras mutations in hyperplasia and carcinoma in cases of human pancreatic carcinoma [J]. Jpn J Cancer Res, 1999, 90(8): 841848.
20.	Okai T, Watanabe H, Yamaguchi Y, et al. EUS and Kras analysis of pure pancreatic juice collected via a duodenoscope after secretin stimulation for diagnosis of pancreatic mass lesion: a prospective study [J]. Gastrointest Endosc, 1999, 50(6): 797803.
21.	Westra WH, Sturm P, Drillenburg P, et al. Kras oncogene mutations in osteoclastlike giant cell tumors of the pancreas and liver: genetic evidence to support origin from the duct epithelium [J]. Am J Surg Pathol, 1998, 22(10): 12471254.
22.	Tateishi K, Tada M, Yamagata M, et al. High proportion of mutant Kras gene in pancreatic juice of patients with pancreatic cystic lesions [J]. Gut, 1999, 45(5): 737740.
23.	Kimura W, Zhao B, Futakawa N, et al. Significance of Kras codon 12 point mutation in pancreatic juice in the diagnosis of carcinoma of the pancreas [J]. Hepatogastroenterology, 1999, 46(25): 532539.
24.	Ebert MP, Hoffmann J, SchneiderStock R, et al. Analysis of Kras gene mutations in rare pancreatic and ampullary tumours [J]. Eur J Gastroenterol Hepatol, 1998, 10(12): 10251029.
25.	Mora J, Puig P, Boadas J, et al. Kras gene mutations in the diagnosis of fineneedle aspirates of pancreatic masses: prospective study using two techniques with different detection limits [J]. Clin Chem, 1998, 44(11): 22432248.
26.	Pabst B, Arps S, Binmoeller K, et al. Analysis of Kras mutations in pancreatic tissue after fine needle aspirates [J]. Anticancer Res, 1999, 19(4A): 24812483.
27.	Singh A, Greninger P, Rhodes D, et al. A gene expression signature associated with “KRas addiction” reveals regulators of EMT and tumor cell survival [J]. Cancer Cell, 2009, 15(6): 489500.

1. Young A, Lyons J, Miller AL, et al. Ras signaling and therapies [J]. Adv Cancer Res, 2009, 102(09): 117.
2. Shaw RJ, Cantley LC. Ras, PI(3)K and mTOR signalling controls tumour cell growth [J]. Nature, 2006, 441(7092): 424430.
3. Tang YT, Hu T, Arterburn M, et al. PAQR proteins: a novel membrane receptor family defined by an ancient 7transmembrane pass motif [J]. J Mol Evol, 2005, 61(3): 372380.
4. Zhu Y, Bond J, Thomas P. Identification, classification, and partial characterization of genes in humans and other vertebrates homologous to a fish membrane progestin receptor [J]. Proc Natl Acad Sci USA, 2003, 100(5): 22372242.
5. Lin SG, Zhang R, Wang JQ. RNA interference technique and the using [J]. J Mod Lab Med, 2008, 23(4): 8386.
6. Bivona TG, Pérez De Castro I, Ahearn IM, et al. Phospholipase cgamma activates Ras on the golgi apparatus by means of RasGRP1 [J]. Nature, 2003, 424(6949): 694698.
7. Rehli M, Krause SW, Schwarzfischer L, et al. Molecular cloning of a novel macrophage maturationassociated transcript encoding a protein with several potential transmembrane domains [J]. Biochem Biophys Res Commun, 1995, 217(2): 661667.
8. de Braud F, Cascinu S, Gatta G. Cancer of pancreas [J]. Crit Rev Oncol Hematol, 2004, 50(2): 147155.
9. Mann O, Strate T, Schneider C, et al. Surgery for advanced and metastatic pancreatic cancer—current state and perspectives [J]. Anticancer Res, 2006, 26(1B): 681686.
10. Riall TS, Nealon WH, Goodwin JS, et al. Pancreatic cancer in the general population: Improvements in survival over the last decade [J]. J Gastrointest Surg, 2006, 10(9): 12121223.
11. Wenger FA, Peter F, Zieren J, et al. Prognosis factors in carcinoma of the head of the pancreas [J]. Dig Surg, 2000, 17(1): 2935.
12. Wakeman CJ, Martin IG, Robertson RW, et al. Pancreatic cancer: management and survival [J]. ANZ J Surg, 2004, 74(11): 941944.
13. Takhar AS, Palaniappan P, Dhingsa R, et al. Recent developments in diagnosis of pancreatic cancer [J]. BMJ, 2004, 329(7467): 668673.
14. Sarkar FH, Banerjee S, Li Y. Pancreatic cancer: pathogenesis, prevention and treatment [J/OL]. Toxicol Appl Pharmacol, 2007, 224(3): 326336.
15. Wright WE, Shay JW, Piatyszek MA. Modifications of a telomeric repeat amplification protocol (TRAP) result in increased reliability, linearity and sensitivity [J]. Nucleic Acids Res, 1995, 23(18): 37943795.
16. Rozenblum E, Schutte M, Goggins M, et al. Tumorsuppressive pathways in pancreatic carcinoma [J]. Cancer Res, 1997, 57(9): 17311734.
17. Chiu VK, Bivona T, Hach A, et al. Ras signalling on the endoplasmic reticulum and the Golgi [J]. Nat Cell Biol, 2002, 4(5): 343350.
18. Hirai H, Okabe T, Anraku Y, et al. Activation of the cKras oncogene in a human pancreas carcinoma [J]. Biochem Biophys Res Commun, 1985, 127(1): 168174.
19. Matsubayashi H, Watanabe H, Yamaguchi T, et al. Multiple Kras mutations in hyperplasia and carcinoma in cases of human pancreatic carcinoma [J]. Jpn J Cancer Res, 1999, 90(8): 841848.
20. Okai T, Watanabe H, Yamaguchi Y, et al. EUS and Kras analysis of pure pancreatic juice collected via a duodenoscope after secretin stimulation for diagnosis of pancreatic mass lesion: a prospective study [J]. Gastrointest Endosc, 1999, 50(6): 797803.
21. Westra WH, Sturm P, Drillenburg P, et al. Kras oncogene mutations in osteoclastlike giant cell tumors of the pancreas and liver: genetic evidence to support origin from the duct epithelium [J]. Am J Surg Pathol, 1998, 22(10): 12471254.
22. Tateishi K, Tada M, Yamagata M, et al. High proportion of mutant Kras gene in pancreatic juice of patients with pancreatic cystic lesions [J]. Gut, 1999, 45(5): 737740.
23. Kimura W, Zhao B, Futakawa N, et al. Significance of Kras codon 12 point mutation in pancreatic juice in the diagnosis of carcinoma of the pancreas [J]. Hepatogastroenterology, 1999, 46(25): 532539.
24. Ebert MP, Hoffmann J, SchneiderStock R, et al. Analysis of Kras gene mutations in rare pancreatic and ampullary tumours [J]. Eur J Gastroenterol Hepatol, 1998, 10(12): 10251029.
25. Mora J, Puig P, Boadas J, et al. Kras gene mutations in the diagnosis of fineneedle aspirates of pancreatic masses: prospective study using two techniques with different detection limits [J]. Clin Chem, 1998, 44(11): 22432248.
26. Pabst B, Arps S, Binmoeller K, et al. Analysis of Kras mutations in pancreatic tissue after fine needle aspirates [J]. Anticancer Res, 1999, 19(4A): 24812483.
27. Singh A, Greninger P, Rhodes D, et al. A gene expression signature associated with “KRas addiction” reveals regulators of EMT and tumor cell survival [J]. Cancer Cell, 2009, 15(6): 489500.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Study on Relationship Between RasTG and Pancreatic Carcinoma

Abstract Full text Figures/Tables Video References Cited by

Format

Content