Surgical Technique of Allogeneic Whole Pancreaticoduodenal Transplantation Model in Rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

1.The First Endemic Area of Department of Hepatobiliary　Pancreatic Surgery, The Second Affiliated Hospital of Kunming Medical College, Kunming 650101, China;;
2.Department of Gastrointestinal Surgery, The First Affiliated Hospital of Kunming Medical College, Kunming 650031, China;;
3.Department of Hepatobiliary Surgery,　Ｔhe First People’s Hospital of Kunming City,Kunming 650011, ChinaCorresponding Author: LI Li, E-mail: ynkmlili@yahoo.com.cn;

Corresponding author：

Keywords：

Pancreaticoduodenal transplantation; Animal model; Rat

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Objective To explore the key technique of allogeneic whole pancreaticoduodenal transplantation (WPDT) in rats. MethodsWPDT model was established between Lewis rats as donors and Wistar rats (with type 1 diabetes mellitus) as recipients. End to side anastomosis was performed in abdominal aorta of donors and recipients. The portal vein of the graft was anastomosed with the recipients left renal vein by cuff technique. And side to side anastomosis was made between the graft duodenum and the host jejunum. ResultsForty-four of 50 rats were successfully performed WPDT. Amongthem, 8 rats died in postoperative 3 days, the survival time of residual 36 rats was 6-16 days, with an average of (10.45±3.30) days. The peak of death appeared on day 7-10 after operation. The typical acute rejection in pathological changes were observed on day 7. ConclusionSkilled microsurgical techniques and emphasis on details are important to establish WPDT model.

Citation： WEI Xiaoping,DIAO Chang,LI Li. Surgical Technique of Allogeneic Whole Pancreaticoduodenal Transplantation Model in Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2009, 16(2): 111-114. doi: Copy

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2.	Gruessner AC, Sutherland DE. Pancreas transplant outcomes for United States (US) and non-US cases as reported to the United Network for Organ Sharing (UNOS) and the International Pancreas Transplant Registry (IPTR) as of June 2004 [Ｊ]. Clin Transplant, 2005; 19(4)：433.
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9.	张召辉，李玺，路逵阳，等.大鼠胰十二指肠移植模型的建立[Ｊ]. 徐州医学院学报， 2003； 23(3)：205.
10.	虞俊波，徐泽宽.肠造瘘式大鼠胰十二指肠移植模型的建立[Ｊ]. 中华实验外科杂志，2006； 23(1)：109.
11.	Jiga LP, Nitǎ G, Dornean V, et al. Experimental model of pancreas transplantation with portal or systemic drainage in the laboratory rat[Ｊ].Chirurgia (Bucur), 2007; 102(5)：563.
12.	陈彦，朱兴国，李德春.RANTES在同种异体胰腺十二指肠移植大鼠中的表达[Ｊ]. 苏州大学学报（医学版）， 2006； 26(1)：29.
13.	Miao G, Ito T, Uchikoshi F, et al. Development of donor-specific immunoregulatory T-cells after local CTLA4Ig gene transfer to pancreatic allograft [Ｊ]. Transplantation, 2004; 78(1)：59.
14.	Yuan CH, Liu YF, Cheng Y, et al. Protective effects of L-arginine on reperfusion injury after pancreaticoduodenal transplantation in rats [Ｊ]. Hepatobiliary Pancreat Dis Int, 2004; 3(3)：349.
15.	Ma Y, Guo ZY. Use of surgical techniques in the rat pancreas transplantation model [Ｊ]. Hepatobiliary Pancreat Dis Int, 2008; 7(2)：156.
16.	刘忠，关风林，沈忠义，等.异基因大鼠全胰十二指肠移植急性排斥反应的病理变化[Ｊ].中国普通外科杂志， 2002； 11(3)：165.
17.	胡伟明，韩方海，张肇达，等.猪同种异体胰十二指肠移植门静脉回流肠内引流模型的实验研究[Ｊ].中国普外基础与临床杂志， 2007； 14(2)：171.
18.	Mark W, Hechenleitner P, Dietze O, et al. Duodenal histology for monitoring treatment of acute rejection in pancreaticoduodenal allografts in rats [Ｊ]. Transplantation, 2002; 73(2)：198.
19.	董高宏，张肇达，胡伟明，等.胰-十二指肠移植肠内引流的新型大动物模型[Ｊ]. 南方医科大学学报， 2006； 26(5)：626.
20.	Gu Y, Li J, Li N. Insulin sensitivity after pancreaticoduodenal transplantation with systemic and portal venous drainage in inbred rats [Ｊ]. Chin Med J (Engl), 2002; 115(4)：549.

1. Ito T, Uchikoshi F, Tori M, et al. Immunological characteristics of pancreas transplantation: review and our experimental experience [Ｊ]. Pancreas, 2003; 27(1)：31.
2. Gruessner AC, Sutherland DE. Pancreas transplant outcomes for United States (US) and non-US cases as reported to the United Network for Organ Sharing (UNOS) and the International Pancreas Transplant Registry (IPTR) as of June 2004 [Ｊ]. Clin Transplant, 2005; 19(4)：433.
3. 夏穗生. 临床肝移植进展中的思考[Ｊ].中华肝胆外科杂志， 2005； 11(11)：721.
4. 郑树森，徐骁.当今胰腺移植的重大进展与前景[Ｊ].中国普外基础与临床杂志， 2003； 10(3)：201.
5. 刁畅，魏晓平，李立，等.双套管+腹主动脉三通管外接法建立大鼠胰十二指肠移植模型[Ｊ].中国普外基础与临床杂志， 2008； 15(4)：254.
6. 刘其雨，程若川，苏艳军，等.双套管法制作大鼠胰十二指肠移植模型[Ｊ].中国普外基础与临床杂志， 2007； 14(4)：420.
7. 朱兴国，田清水，陈彦，等.大鼠胰十二指肠移植动物模型的制作[Ｊ]. 中华实验外科杂志， 2005； 22(4)：496.
8. 王为忠，刘小南，李开宗，等. 大鼠单纯胰腺移植手术技术[Ｊ]. 中国普外基础与临床杂志， 2005； 12(2)：147.
9. 张召辉，李玺，路逵阳，等.大鼠胰十二指肠移植模型的建立[Ｊ]. 徐州医学院学报， 2003； 23(3)：205.
10. 虞俊波，徐泽宽.肠造瘘式大鼠胰十二指肠移植模型的建立[Ｊ]. 中华实验外科杂志，2006； 23(1)：109.
11. Jiga LP, Nitǎ G, Dornean V, et al. Experimental model of pancreas transplantation with portal or systemic drainage in the laboratory rat[Ｊ].Chirurgia (Bucur), 2007; 102(5)：563.
12. 陈彦，朱兴国，李德春.RANTES在同种异体胰腺十二指肠移植大鼠中的表达[Ｊ]. 苏州大学学报（医学版）， 2006； 26(1)：29.
13. Miao G, Ito T, Uchikoshi F, et al. Development of donor-specific immunoregulatory T-cells after local CTLA4Ig gene transfer to pancreatic allograft [Ｊ]. Transplantation, 2004; 78(1)：59.
14. Yuan CH, Liu YF, Cheng Y, et al. Protective effects of L-arginine on reperfusion injury after pancreaticoduodenal transplantation in rats [Ｊ]. Hepatobiliary Pancreat Dis Int, 2004; 3(3)：349.
15. Ma Y, Guo ZY. Use of surgical techniques in the rat pancreas transplantation model [Ｊ]. Hepatobiliary Pancreat Dis Int, 2008; 7(2)：156.
16. 刘忠，关风林，沈忠义，等.异基因大鼠全胰十二指肠移植急性排斥反应的病理变化[Ｊ].中国普通外科杂志， 2002； 11(3)：165.
17. 胡伟明，韩方海，张肇达，等.猪同种异体胰十二指肠移植门静脉回流肠内引流模型的实验研究[Ｊ].中国普外基础与临床杂志， 2007； 14(2)：171.
18. Mark W, Hechenleitner P, Dietze O, et al. Duodenal histology for monitoring treatment of acute rejection in pancreaticoduodenal allografts in rats [Ｊ]. Transplantation, 2002; 73(2)：198.
19. 董高宏，张肇达，胡伟明，等.胰-十二指肠移植肠内引流的新型大动物模型[Ｊ]. 南方医科大学学报， 2006； 26(5)：626.
20. Gu Y, Li J, Li N. Insulin sensitivity after pancreaticoduodenal transplantation with systemic and portal venous drainage in inbred rats [Ｊ]. Chin Med J (Engl), 2002; 115(4)：549.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Surgical Technique of Allogeneic Whole Pancreaticoduodenal Transplantation Model in Rats

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