【Abstract】ObjectiveTo investigate the effect of RNA editing enzyme ADAR1 on the function of lymphocyte immune by transferring mouse lymphocytes with plasmid of sense siRNA and by suppressing the expression of ADAR1.
MethodsThe cell strains of human hepatic cellular carcinoma （HCC） were frozen and thawed repeatedly to prepare for tumor soluble antigen. The isolated mouse lymphocytes, which were transferred with antisense siRNA plasmid of ADAR1 and were sensitized with soluble tumor antigen were used as the study group; those which were not transferred but were sensitized were used as the control group. The 3HTdR adulteration experiment was used to test the sensitivity of lymphocytes. The effect of ADAR1 on lymphocyte immunity was detected by lymphocytotoxicity tests.
ResultsThe observation of the isolated lymphocytes implied that the growth cycle of lymphocyte was 10-14 days. The 3HTdR adulteration experiment showed the result was optimal. The number of HCCs decreased significantly for both of the groups compared with those in the blank holes, but the amplitude was much larger in the control group. The expression of ADAR1 in lymphocytes of the study group was significantly lower than that of the control group, which demonstrated that the RNA plasmid of ADAR1 suppressed the expression of ADAR1 in sensitized lymphocytes and the suppressing rate of the control group (87.47±4.62)% was significantly higher than that of study group （53.19±3.95）%. The function of lymphocytes killing targetcells in the study group was significantly inferior to that of control group (P＜0.05).
ConclusionRNA editing enzyme ADAR1 may play an important role in mouse cellullar immunologic response and it is possible to attenuate the cellimmune response by depressing the expression of ADAR1.
Citation：
KONG Yalin,DOU Kefeng,FENG Quanxin,ZHANG Hongtao,LI Jun,ZHANG Fuqin,ZHAO Qingchuan.. Function of RNA Editing Enzyme ADAR1 in Cellullar Immunologic Response in Mouse. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2006, 13(3): 279-282. doi:
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Copyright © the editorial department of CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY of West China Medical Publisher. All rights reserved
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Rabinovici R, Kabir K, Chen M, et al. ADAR1 is involved in the development of microvascular lung injury [Ｊ]. Circ Res，2001; 88（10）∶1066.
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孔亚林， 张洪涛， 赵青川， 等. 共培养体系中小鼠淋巴细胞RNA编辑酶的表达 [Ｊ]. 第四军医大学学报， 2006； 27（5）∶392.
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- 1. [Ｊ]. Nature, 1995; 374(6517)∶ 77.
- 2. 〖HJ*3〗8Sprengel R, Higuchi M, Monyer H, et al. Glutamate receptor channels: a possible link between RNA editing in the brain and epilepsy [Ｊ]. Adv Neurol, 1999; 79(2)∶525.
- 3. Yang JH, Sklar P, Axel R, et al. Editing of glutamate receptor subunit B premRNA in vitro by sitespecific deamination of adenosine.
- 4. Zhao QC, Zhang DX, Luo XX, et al. Ｃhangs of RNA editase ADAR1 during mouse primary lymphocyte proliferation [Ｊ]. Chin J Cell Mol Immunol, 2002; 18(3)∶208.
- 5. 常连庆， 谢晓华， 陈雯， 等. 螺内酯抗钙调神经磷酸依赖的肾性高血压大鼠心肌肥厚作用机制 [Ｊ].中华心血管病杂志， 2004; 32(3)∶254.
- 6. 赵素兰， 郭英， 焦艳， 等. 成纤维突变株的实验研究 [Ｊ]. 中华实验与病毒学杂志, 1997; 11（3）∶290.
- 7. Mmer B, Kohler M, Sprengel R, et al. RNA editing in brain controls a determinant of ion flow in glutamategated channels [Ｊ]. Cell， 1991; 67（1）∶11.
- 8. Keegan LP, Gallo A, O’Connell MA. Development. Survival is impossible without an editor [Ｊ]. Science， 2000; 290（5497）∶1707.
- 9. Haub M, Keller W. RNA editing by adenosine deaminases generates RNA and protein diversity [Ｊ]. Biochimie, 2002; 84（8）∶791.
- 10. Kortenbruck G, Berger E, Speckmann EJ, et al. RNA editing at the Q/R site for the glutamate receptor subunits GLUR2, GLUR5, and GLUR6 in hippocampus and temporal cortex from epileptic patients [Ｊ]. Neurobiol Dis， 2001; 8（3）∶459.
- 11. Patterson JB, Thomis DC, Hans SL, et al. Mechanism of interferon action: doublestranded RNAspecific adenosine deaminase from human cells is inducible by alpha and gamma interferons [Ｊ]. Virology， 1995; 210(2)∶508.
- 12. Rabinovici R, Kabir K, Chen M, et al. ADAR1 is involved in the development of microvascular lung injury [Ｊ]. Circ Res，2001; 88（10）∶1066.
- 13. Zhao QC, Zhang DX, Luo XX, et al. Ｃhangs of RNA editase ADAR1 during mouse primary lymphocyte proliferation [Ｊ]. Chin J Cell Mol Immunol, 2002; 18(3)∶208.
- 14. 孔亚林， 张洪涛， 赵青川， 等. 共培养体系中小鼠淋巴细胞RNA编辑酶的表达 [Ｊ]. 第四军医大学学报， 2006； 27（5）∶392.
