【Abstract】ObjectiveTo investigate the proliferation rate of HepG2 cell after multiple thermotherapy and the possible reasons related to it.
MethodsAfter HepG2 cell were treaded by ten repeated cycles of heat exposure at 43 ℃ for 80 minutes twice a day, the doubling time of cell was analyzed， and the cell cycle, bcl2 mRNA and bax mRNA were detected.
ResultsThe proliferation rate of HepG2 cell which treated with heat speeded up, the percentage of G2 and S in cell cycle increased, the expression of bcl2 mRNA strengthened and the rate of bcl2/bax increased.
ConclusionThe speeded proliferation of HepG2 cell after multiple thermotherapy is related to its high percentage of DNA duplicated and dividing cell, strengthened expression of bcl2 mRNA and increased rate of bcl2/bax.
Citation：
QIN Chunhong,LI Yongguo.. Effect of Multiple Thermotherapy on Proliferation Cycle and bcl-2 mRNA of HepG2 Cell. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2006, 13(3): 295-297下转304. doi:
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- 2. Beierle EA, Dai W, Iyengar R, et al. Differential expression of bcl2 and bax may enhance neuroblastoma survival [Ｊ]. J Pediatr Surg, 2003; 38(3)∶486.
- 3. Pettersson F, Dalgleish AG, Bissonnette RP, et al. Retinoids cause apoptosis in pancreatic cancer cells via activation of RARgamma and altered expression of bcl2/bax [Ｊ]. Br J Cancer, 2002; 87(5)∶555.
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