Effects of Glutamine Combined with Growth Hormone on Cytokine and Amino Acid Metabolism in Septic Rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

LIU Minfeng , GAO Zhiguang , QIN Huanlong.

Department of Surgery, Shanghai Jiaotong University, Affiliated the Sixth People’s Hospital, Shanghai 200233, China;

Corresponding author：

Keywords：

SepsisAmino acidGlutamineGrowth hormoneCytokine

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【Abstract】　Objective　To study the effects of glutamine (Gln) combined with growth hormone （GH） on the levels of cytokine (TNF-α, IL-1, IL-6), coritsol and amino acid metabolism in septic rats. Methods　Ten out of 79 SD rats were randomly collected as the control group. Thirty of 69 septic SD rats, which were made by cecal ligation and perforation (CLP) method and were given parenteral nutrition (PN) lived to day 6. They were also randomly divided into three groups as follows: septic group (n=10), parenteral supplemented glutamine group (Gln group, n=10), and Gln combined with GH (Gln+GH group, n=10). On the 6th day, blood drew from portal veins of the dead rats was used to detect the levels of TNF-α， IL-1， IL-6 and cortisol by ELISA. The plasma concentrations of free amino acids were determined by amino acid auto-analyzer. The muscle tissue of extensor digitorum longus was used to determine 3-methyl-histidine (3-MH) by high performance liquid chromatographic (HPLC). Results　 Except for the control group, most rats developed celiac abscess, hepatic abscess and pulmonary infection. The serum levels of TNF-α, IL-1, IL-6 and cortisol were significantly higher in the septic group than those of the other three groups, and they were significantly lower in the Gln+GH group than those of the Gln group, P＜0.05. Compared with the other three groups, the concentration of total amino acid in the septic group increased more, among which the glutamine and the branched chain amino acids were prominent. Most of concentrations of the amino acids decreased in the Gln group and the Gln+GH group, and the decreased amplitude of the Gln+GH group was larger, P＜0.05, albeit its level of Gln markedly increased. The concentration of 3-MH in muscle tissue was the highest in septic group, and it was significantly higher in the Gln group than that of the Gln+GH group, P＜0.01. Conclusion
It may be necessary to supplement GH combined with Gln as the content of PN to decrease cytokine levels and im-prove amino acid metabolism for septic case.

Citation： LIU Minfeng,GAO Zhiguang,QIN Huanlong.. Effects of Glutamine Combined with Growth Hormone on Cytokine and Amino Acid Metabolism in Septic Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2006, 13(5): 535-539. doi: Copy

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11.	卢才教, 林　才，徐建军, 等. 联合应用谷氨酰胺和重组人生长激素对严重烧伤患者蛋白代谢的影响 [J]. 中华烧伤杂志， 2004; 20（4）∶220.

1. 　Costelli P, Bossola M, Muscaritoli M, et al. Anticytokine treatment prevents the increase in the activity of ATP-ubiquitin-and Ca(2+)-dependent proteolytic systems in the muscle of tumour-bearing rats [J]. Cytokine, 2002; 19(1)∶1.
2. 　刘敏丰，秦环龙. 外科危重疾病时肌肉恶病质的发生机制研究进展 [J]. 中国危重病急救医学杂志, 2004; 16（11）∶695.
3. 　Steiger E, Vars HM, Dudrick SJ. A technique for long-term intravenous feeding in unrestrained rats [J]. Arch Surg, 1972; 104(3)∶330.
4. 　Lidington D, Ouellette Y, Li F, et al. Conducted vasoconstriction is reduced in a mouse model of sepsis [J]. J Vasc Res, 2003; 40(2)∶149.
5. 　沈通一, 秦环龙，佟大年, 等. 肠外肠内营养对腹腔感染大鼠肠上皮紧密连接和屏障功能的影响 [J]. 中华普通外科杂志， 2005; 20(3)∶174.
6. 　吴焱秋，柴家科，黎君友, 等. 大鼠严重烫伤早期血浆游离氨基酸浓度的变化 [J]. 中华烧伤杂志， 2001; 17（4）∶215.
7. 　Okamura K, Okuma T, Tabira Y, et al. Effect of administered human growth hormone on protein metabolism in septic rats [J]. JPEN J Parenter Enteral Nutr, 1989; 13(5)∶450.
8. 　Gamrin L, Essen P, Hultman E, et al. Protein-sparing effect in skeletal muscle of growth hormone treatment in critically ill patients [J]. Ann Surg, 2000; 231(4)∶577.
9. 　Millward DJ, Jepson MM, Omer A. Muscle glutamine concentration and protein turnover in vivo in malnutrition and in endotoxemia [J]. Metabolism, 1989; 38(8 Suppl 1)∶6.
10. 　桑剑锋，吴文溪，许　勤, 等. 添加丙氨酰谷氨酰胺胃肠外营养对烫伤大鼠蛋白质代谢的作用 [J]. 现代医学， 2003; 31（6）∶362.
11. 　卢才教, 林　才，徐建军, 等. 联合应用谷氨酰胺和重组人生长激素对严重烧伤患者蛋白代谢的影响 [J]. 中华烧伤杂志， 2004; 20（4）∶220.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effects of Glutamine Combined with Growth Hormone on Cytokine and Amino Acid Metabolism in Septic Rats

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