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find Keyword "乙型肝炎病毒" 39 results
  • Drugs and hepatitis B virus reactivation

    Drugs may induce hepatitis B virus (HBV) reactivation (HBV-R). Here we have reviewed the definition and harm of HBV-R, the risk drugs and their underlying mechanism, the influence factors, as well as the early intervention measures. It is shown that multiple drugs, including chemotherapy drugs, immunotherapy drugs, directly acting antivirals, cell therapy, etc., can induce HBV-R by affecting host immunity or directly activating HBV transcription factors. HBV-R could cause severe liver damage, even interruption of treatment of original diseases, affecting the prognosis of patients. Through precisely identifying risk drugs, monitoring the influence factors, and prescribing preventive anti-HBV regimen if necessary, the incidence of HBV-R can be significantly reduced. It is also suggested that clinical physicians should not only pay attention to the early identification and intervention of HBV-R, but also further study the mechanism of HBV-R in depth, especially the underlying mechanism between host, HBV and risk factors. This will help to promote the discovery of more valuable markers for risk prediction and targets for early intervention, and to further reduce the risk of HBV-R and improve the prognosis of patients.

    Release date:2022-08-22 03:12 Export PDF Favorites Scan
  • CRISPR/Cas9 技术在乙型肝炎病毒基因组抑制中的应用

    目前世界范围内约有 2.4 亿慢性乙型肝炎病毒(hepatitis B virus,HBV)感染者,HBV 感染是世界性的重大公共卫生难题。随着分子生物学工具的不断发展,目前第 3 代基因定点编辑技术 CRISPR/Cas9 作为热点已经广泛地应用于多种病毒的研究与实验性治疗中。该文简要回顾了 HBV 基因组的特点、基因编辑技术的发展及原理和 CRISPR/Cas9 在 HBV 基因组抑制中的研究现状及局限性。相对于锌指核糖核酸酶和转录激活因子样效应物核酸酶其他两种基因编辑技术,CRISPR/Cas9 技术极大地提高了基因编辑的能力。虽然目前仍属于概念证明阶段,但多数基础研究均证实了 CRISPR/Cas9 技术在体内外对 HBV 基因组具有编辑能力并能降低其 DNA 复制与病毒蛋白的表达能力。在潜在安全风险及基因编辑载体的输送效率等问题得到解决后,CRISPR/Cas9 技术联合逆转录抑制药物的治疗将为 HBV 感染的临床治愈带来曙光。

    Release date:2017-12-25 06:02 Export PDF Favorites Scan
  • Clinical characteristics analysis of hepatitis B virus infected individuals with high hepatitis B virus pregenomic RNA

    Objective To analyze the clinical characteristics of individuals with high hepatitis B virus (HBV) pregenomic RNA (pgRNA), and further explore the value of pgRNA in the management of patients with chronic hepatitis B. Methods From December 1st, 2020 to April 1st, 2022, chronic hepatitis B patients who had been treated with nucleotide analogues for a long time and followed up in the Hepatitis Clinic of the Center of Infectious Diseases, West China Hospital, Sichuan University were included, and the clinical characteristics of chronic hepatitis B patients with high pgRNA were analyzed and summarized. Results A total of 107 patients were included. Male patients accounted for 66.4%, with an average age of 44.02 years. There were no statistically significant differences in gender, age, aspartate transaminase, alanine transaminase, γ-glutamyl transferase, HBV surface antigen, proportion of patients with HBV e antigen ≥0.1 U/mL, HBV DNA, and alpha fetoprotein between the high and low pgRNA groups (P>0.05). The proportion of patients with HBV surface antigen<100 U/mL in the high pgRNA group was lower than that in the low pgRNA group (4.4% vs. 22.6%, P<0.05). Conclusion The proportion of chronic hepatitis B patients with high pgRNA whose HBV surface antigen≥100 U/mL is higher.

    Release date:2024-09-23 01:22 Export PDF Favorites Scan
  • 微 RNA 在乙型肝炎相关疾病中作用研究进展

    乙型肝炎病毒(hepatitis B virus,HBV)感染是造成肝纤维化、肝衰竭和肝癌的主要原因。微 RNA(microRNA,miRNA)在 HBV 慢性感染状态下出现异常表达。部分表达异常的 miRNA 可以通过活化肝星状细胞或产生胶原蛋白来促进肝纤维化过程;也可以通过诱导肝细胞短期内大量坏死或凋亡来加速肝衰竭进展;亦可以通过上调癌基因或下调抑癌基因表达促进癌症的发生与发展。该文初步探讨了 miRNA 在肝纤维化、肝衰竭、肝癌中的作用。

    Release date:2017-11-24 10:58 Export PDF Favorites Scan
  • 慢性乙型肝炎病毒感染者检测血清乙型肝炎病毒

    【摘要】 目的 观察慢性乙型肝炎病毒(hepatitis B virus,HBV)感染者血清中HBV共价闭合环状DNA(covalently closed circular DNA,cccDNA)的分布特点及其与不同疾病状态的关系。 方法 2008年1月-12月收治慢性HBV感染者120例,男79例,女41例;年龄15~52岁,平均35岁。其中慢性HBV携带者21例,HBeAg阳性者38例,HBeAg阴性者35例,非活动性HBsAg携带者26例。采用巢式PCR法检测血清中HBV cccDNA。 结果 120例慢性HBV感染者血清中HBV cccDNA阳性总检出率为43.3%;慢性HBV携带者、HBeAg阳性者、HBeAg阴性和非活动性HBsAg携带者cccDNA阳性检出率分别为76.2%、64.7%、34.3%和0,各组间比较差异有统计学意义(Plt;0.05)。血清高HBV DNA定量组HBV cccDNA阳性检出率高于低HBV DNA定量组(Plt;0.05)。结论 HBV cccDNA检出率与外周血HBV复制指标HBeAg、HBV DNA有显著的相关性,并与不同疾病状态相关。

    Release date:2016-09-08 09:31 Export PDF Favorites Scan
  • 重组聚合酶链反应扩增乙型肝炎病毒跨直接重复序列区DNA片段方法的建立

    目的建立使用重组聚合酶链反应(PCR)扩增乙型肝炎病毒(HBV)跨直接重复序列(DR)区DNA 片段的方法。 方法使用Primer5 引物设计软件,以黏性末端为基础设计引物,HBV“大三阳”乙型肝炎表面抗原(+)、乙型肝炎核心抗体(+)、乙型肝炎e 抗原HBeAg(+)] 血清提取DNA 为PCR 模板,第1 轮PCR 分段扩增,第2 轮PCR 以粘性末端为引物两端补齐,第3 轮PCR 扩增整段HBV 跨DR 区DNA 片段。 结果成功重组出HBV跨DR 区缺口的DNA 片段。 结论建立了HBV 跨DR 区DNA 片段的扩增方法,为该段DNA 片段功能的研究打下了基础。

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  • Efficacy of Antiviral Drugs for Hepatitis B with YMDD Motif Variant: A Systematic Review

    Objective To evaluate the efficacy and safety of antiviral drugs for hepatitis B with YMDD motif variant. Methods We electronically searched MEDLINE (1989-April, 2004), EMBASE (1989-April, 2004), CBMdisc (expand) (1989-April, 2004), and handsearched unpublished Chinese conference proceedings. Randomized and quasi-randomized trials in patients with chronic hepatitis B with YMDD motif variant correlative to lamivudine were collected. Two reviewers extracted the data and assessed the quality of literature independently. The data were then analyzed by RevMan 4.2 software. Results Five studies involving 6 trials and 284 patients were included. According to the results of meta-analysis, antiviral therapy with adefovir plus lamivudine showed significantly better effects on the clearance of serum HBV-DNA and HBeAg and normalization of ALT than that of lamivudine alone (RR 16.61, 95%CI 2.29 to 120.71; RR 6.66, 95%CI 1.23 to 35.88 and RR 6.26, 95%CI 2.29 to 17.12 respectively); also, oxymatrine plus thymothin showed obviously better effects on the clearance of serum HBV-DNA and HBeAg (RR 2.96, 95%CI 1.26 to 6.93 and RR 2.51, 95%CI 1.05 to 5.98 respectively).But adefovir alone showed no better effects on clearance of serum HBV-DNA and HBeAg than that of lamivudine alone (RR 11.00, 95%CI 0.65 to 186.02 and RR 7.00, 95%CI 0.39 to 126.92 respectively); interferon plus lamivudine showed no better effects on the clearance of serum HBV-DNA, HBeAg and the normalization of ALT (RR 3.50, 95%CI 0.90 to 13.58; RR 4.90, 95%CI 0.70 to 35.10 and RR 2.80, 95%CI 0.91 to 8.12 respectively). Chinese herbs plus lamivudine showed no better effects on the clearance of serum HBV-DNA (RR 1.16, 95%CI 0.89 to 1.51). There were no significant side effects in the groups, except flu like symptom in the interferon group, slight kidney impairment in the adefovir group, and aggravation of rare cases in lamivudine group. Conclusions Antiviral therapy with adefovir plus lamivudine, or oxymatrine plus thymothin, shows better effects than with lamivudine alone in terms of antiviral therapy and clinical outcome improvement. However, the evidence is too weak to draw a definite conclusion in this systematic review. Larger sample size and rigorously designed randomized, double blind, placebo control trials are required for future study.

    Release date:2016-09-07 02:27 Export PDF Favorites Scan
  • 检测慢性HBV血清HBVcccDNA的临床意义

    【摘要】 目的 观察HBV阳性患者血清中HBV共价闭合环状DNA(cccDNA)的检出率及其与不同疾病状态的关系。 方法 选取2008年1月-12月收治的慢性HBV感染者120例,其中慢性HBV携带者21例,HBeAg阳性慢性乙型肝炎38例,HBeAg阴性慢性乙型肝炎35例,非活动性HBsAg携带者26例。采用巢式PCR法检测全部患者血清中的HBV cccDNA。 结果 120例血清中HBV cccDNA阳性检出率为43.3%(52/120);慢性HBV携带者、HBeAg阳性慢性乙型肝炎、HBeAg阴性慢性乙型肝炎和非活动性HBsAg携带者的cccDNA阳性检出率分别为76.2%(16/21)、63.2%(24/38)、34.3%(12/35)和0%,各组间的差异均有统计学意义(Plt;0.05);血清高HBV DNA定量组的HBV cccDNA阳性检出率高于低HBV DNA定量组(Plt;0.05)。 结论 HBV cccDNA检出率与外周血HBV复制指标HBV DNA有关,并与不同疾病状态相关。

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  • Research progress of biomarkers of hepatitis B virus and clinical significance

    The infection of Hepatitis B virus (HBV) can result in severe consequences, including chronic hepatitis, liver fibrosis, cirrhosis, and even liver cancer. Effective antiviral treatment has the potential to slow down the progression of the disease. HBV serum biomarkers play a crucial role in the dynamic management of chronic hepatitis B (CHB) patients. However, the conventional hepatitis B virus markers, such as hepatitis B serologic testing and HBV DNA, are insufficient to meet the clinical requirements. This review provided a comprehensive overview of the current research on the quantification of HBsAg and anti-HBc, HBV RNA and HBV core-associated antigen, which summarized the crucial role these markers play in the administration of antiviral medications, predicting the efficacy of treatment and anticipating the likelihood of virologic rebound following drug cessation, as well as assessing disease progression in CHB patients.

    Release date:2023-12-21 03:53 Export PDF Favorites Scan
  • Influence of Paternal Serum HBV-DNA Load Levels and Pregnant Women's HBsAb on Paternal Vertical Transmission of Hepatitis B Virus

    ObjectiveTo explore the influence of paternal serum HBV-DNA load levels and pregnant women's HBsAb on vertical transmission of hepatitis B virus (HBV) from HBsAg positive fathers to infants in order to provide effective methods for paternal-fetal ventrical transmission of HBV prevention. MethodsUsing HBsAg and HBV-DNA as indicators to screen pregnant women and their husbands after gained consent, 121 families with HBVM negative or only HBsAb positive and HBV-DNA negative pregnant women, HBsAg positive husbands and their newborns were selected. In this case-control study, according to neonatal cord blood HBV-DNA detection, 23 newborns with cord blood HBV-DNA positive were selected as cases, 98 newborns as controls. ResultsThe positive rate of neonatal cord blood HBV-DNA was 19.0% (23/121); and there was dose-response relationship between paternal serum HBV-DNA load levels and neonatal cord blood HBV-DNA positive (trend χ2=60.108, P=0.000). The analysis of ROC curve showed that paternal serum HBV-DNA load level (106 copies/mL) is a better demarcation point to forecast the occurrence of vertical transmission of HBV from HBsAg positive fathers to infants, because there was a better sensitivity and specificity during forecast; and HBsAb negative pregnant women's were statistically significant (χ2=12.399, P=0.000). There was no significant difference at the positive rate of neonatal cord blood HBV-DNA between the case group and control group when paternal serum HBV-DNA load levels exceed 107 copies/mL (P > 0.05). ConclusionPaternal serum HBV-DNA load levels and HBsAb negative pregnant women are the risk factors of vertical transmission of HBV from HBsAg positive father to infants. Paternal serum HBV-DNA load level (106 copies/mL) is an appropriate index of the occurrence of vertical transmission.

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