[Abstract]Chronic obstructive pulmonary disease (COPD) occurs mostly in middle-aged and elderly people, and patients often have poor quality of life and can also induce a variety of other lung diseases. At the same time, the lung cancer with the highest morbidity and mortality in the world, has claimed the lives of countless patients. Since the incidence of lung cancer is much higher in patients with COPD than in the general population, there are many links between the two diseases that need to be studied and explored. Although many studies have explored the association between the two, there are few studies on the molecular level of them. This review will focuse on the latest research progress of the pathogenesis of COPD complicated with lung cancer from four aspects: the role of chronic inflammation, programmed cell death, gene and molecular role and lung microbiome imbalance, so as to provide a new reference for the prevention, diagnosis and treatment of COPD complicated with lung cancer.
In recent years, the application of the long read single-molecule sequencing technology in clinical genetics testing has been increasingly widespread. Due to its sequencing process not requiring amplification and its characteristic of single-molecule sequencing and long reads, it has unique advantages compared to traditional molecular genetics testing such as Sanger sequencing, next-generation sequencing and chromosomal microarray. In this article, its application in the detection of chromosomal structural abnormalities, monogenic diseases, and preimplantation genetic testing for monogenic diseases were discussed. Along with a comparative analysis of the advantages and disadvantages of the long read single-molecule sequencing technology in these areas compared to traditional molecular genetics testing techniques. This article preliminarily explores the application prospects and value of the long read single-molecule sequencing technology in clinical genetics testing.