目的：观察利多卡因宫旁阻滞联合宫腔灌注在人流术中镇痛的效果。方法:收集我院2005年1～2月门诊终止妊娠的早孕妇女共94例并分为两组:单纯宫旁阻滞组（简称单纯组）35例，宫旁阻滞联合宫腔灌注（简称联合组）59例。比较两种利多卡因局麻方式的效果。结果:（1)两组的止痛效果无明显差异(P=0.893);(2)联合组对宫颈的松弛作用优于宫旁阻滞(Ｐ=0.000);(3)联合组较多病例出现眩晕、耳鸣等副反应。结论:与单纯宫旁阻滞相比，宫旁阻滞联合宫腔灌注并无明显优势。
Objective To investigate the effect of N-methyl-N-nitrosourea (MNU) on expressions of interphotoreceptor retinoid-binding protein (IRBP) and recoverin in rat retinal. Methods Thirty female SpragueDawley (SD) rats were randomly divided into five groups, including normal control group, and MNU treatment one day, three days, seven days and 10 days groups, each group had six rats. Rats in MNU-treatment groups received a single intraperitoneal injection of 40 mg /kg MNU, while rats in the normal control group received intraperitoneal injection of saline 5 ml/kg. Reverse transcription-polymerase chain reaction (RT-PCR) and immunofluorescence were used to detect the retinal expression of IRBP and recoverin. Results RT-PCR results indicated that retinal IRBP levels decreased after MNU injection compared with normal control (P＜0.01)，while recoverin levels increased (one day: P＞0.05; three days: P＜0.05; seven days: P＜0.5; 10 days：P＜0.05). Immunofluorescence assays demonstrated that normally IRBP protein is mainly in the inner and outer segments of photoreceptors. After MNU treatment, IRBP protein was detected in all retinal layers but much faint. Recoverin was expressed in the inner nuclear layer, inner plexiform layer and ganglion cell layer, and its expression was increased after MNU injection. Conclusion MNU suppresses IRBP expression and promotes recoverin expression in rat retina.