目的 改善左半结肠癌性梗阻一期切除吻合术中结肠灌洗的方法。方法 利用市售塑料自来水三通管及大口径(直径3 cm)螺纹管制成结肠灌洗器，于梗阻近端结肠置入形成术中封密式结肠灌洗。结果 24例患者采用自制结肠灌洗器行术中结肠灌洗，无腹腔污染，术后无一例发生吻合口漏，均一期愈合，顺利出院。结论 掌握好一期手术适应证，自制结肠灌洗器可运用于左半结肠癌性梗阻一期切除吻合，术中肠腔灌洗彻底，方便，无污染，无溢漏，且制作简单，成本低。
目的 确定不同的经肛引流方式对左半结肠癌患者一期术后外周血浆内毒素(ET)及肿瘤坏死因子(TNF)水平的影响。 方法左半结肠癌伴梗阻患者按术后经肛引流方法不同分为扩肛组(A)、经肛吻合口上单管引流组(B)及经肛吻合口上下双管引流组(C)3个组，观察各组患者一期术后ET及TNF的变化。 结果 术后A、B、C 3组ET及TNF水平均呈下降趋势，与术前比较差异有显著性意义(P＜0.01)。自术后第4天，C组患者ET及TNF水平开始显著低于B组(P＜0.05)。结论 左半结肠癌性梗阻患者一期手术行经肛引流能更有效地减少内毒素的吸收，降低TNF水平，而经肛双管引流法引流效果又优于经肛单管引流法。
ObjectiveTo explore the expressions of polo-like kinase 1(PLK1) and serine/threonine kinase 15 (STK15) mRNA and protein in colon cancer cells, and to explore the inhibitive effect of SBE13 and VX-680 for PLK1 protein and STK15 protein. MethodsOne kind of cervical cancer cells(Hela cells) and 3 kinds of colon cancer cells (HCT-116 cells, HT-29 cells, and CACO-2 cells) were selected for experiment. Expression levels of PLK1 mRNA, STK15 mRNA and its protein of 4 kinds of cells were detected by reverse transcription polymerase chain reaction(RT-PCR) and Western blot method respectively. Inhibitive effect of SBE13 and VX-680 were evaluated in vitro by methylthiazolyldiphenyl-tetrazolium bromide(MTT) assay in 4 kinds of cells, which divided into 5 groups, receiving Dulbecco's modification of Eagle's medium(DMEM), dimethylsulfoxide(DMSO), SBE13, VX-680, and SBE13+VX-680 respectively. ResultsCompared with Hela cells, expression levels of PLK1 mRNA, STK15 mRNA and its protein in HCT-116 cells,HT-29 cells, and CACO-2 cells were higher(P<0.05). ① Hela cells:Compared with DMEM group, the proliferative activity were not inhibited in SBE13 group, VX-680 group, and SBE13+VX-680 group(P>0.05). ② HCT-116 cells and HT-29 cells:Compared with DMEM group, the proliferative activity were inhibited in VX-680 group and SBE13+VX-680 group(P<0.05), but was not inhibited in SBE13 group(P>0.05). ③ CACO-2 cell:Compared with DMEM group, the proliferative activity were inhibited in SBE13 group, VX-680 group, and SBE13+VX-680 group(P<0.05). ConclusionsExpression levels of PLK1 mRNA, STK15 mRNA and its protein increase in HCT-116, HT-29, and CACO-2 cells compared with Hela cells. SBE13 and VX-680 can inhibit PLK1 and STK15 protein partly in colon cancer cell lines.
Objective To investigate the surgical techniques for management of left colon carcinoma obstruction. MethodsThe techniques used in left colon carcinoma obstruction and the corresponding efficiencies reported in foreign literatures were reviewed. ResultsThe surgical techniques for management of left colon carcinoma obstruction involve palliative operation, staged operation and onestage operation. These methods had their own virtues as well as shortages. But on all accounts, onestage operation was favorable for both the patients and docters. ConclusionDifferent methods for management of left colon carcinoma obstruction have different adaptation index, first you must ensure safety, then you should try your best to do onestage operation.