Wistar rats weaned were raised through 10 weeks under cyclic illumination of 12 hours light and 12 hours darkness,with four different fluorescent colour lighting condition:75 lx and 300lx blue light,300 lux white and 300lux pink light to study the change of superoxide dismutases(SOD)and lipid peroxied(LPO)in the retina.This paper shows that photic oxidative reaction reduces SOD in the retina and oxidizes polyunsaturated fatty acids to become LPO and that complex visible light oxidizes retina easier than simple wave lengths visible light does.The shorter the wave lengths of visible light is and the brighter the illumination is the more serious the oxidative damage of the retina is. （Chin J Ocul Fundus Dis,1993,9:14-16）
目的：探讨在不同年龄SD大鼠右心房注射前列腺素E2（PGE2）对呼吸的影响。方法：7～9 d和21～23 d大鼠在迷走神经完整和迷走神经切断的情况下从右心房注射PGE-2，观察呼吸指标的变化。结果：①右心房注射PGE2在7～9 d和21～23 d大鼠中均引起呼吸暂停，呼气延长时间分别为基础呼气时间的9.5和7.5倍（Plt;0.05）；②切断迷走神经后，右心房注射PGE-2在7～9 d和21～23 d大鼠均不再产生呼吸暂停，仅出现轻微呼吸抑制。结论：右心房注射PGE2在7～9 d和21～23 d大鼠均产生呼吸暂停，且依赖于迷走神经的完整性。
目的:探讨宫颈癌体外放射治疗加后装腔内治疗的疗效和放射反应。方法:对76例宫颈癌患者均采用体外放射治疗加60Co 后装腔内治疗临床资料进行回顾性分析。结果:全组 5年总生存率为 63.7%,Ⅰ期生存率为88.9%,Ⅱ期为 71.9%,Ⅲ期为48.1%,Ⅳ期为 20.0%。10年总生存率为 31.6%,Ⅰ期生存率为 53.3%,Ⅱ期为33.3%,Ⅲ期为 22.0%,Ⅳ期为 0.0%,经Log Rank法检验各期生存率有显著差异(Plt;0.01)。放射治疗后发生阴道粘连 9例(11.8%),放射性膀胱炎 6例(7.9%),放射性直肠炎13例(17.1%)。放射性直肠炎按 RTOG 晚期放射损伤分级方案分级,1级7例(53.8%),2级4例(30.8%),3级 2例(15.4%),4级 0例(0.0%)。结论:体外放射治疗加60Co后装腔内治疗宫颈癌疗效肯定,副作用少。但如何进一步提高生存率,尽可能减少放射反应仍是今后研究的方向。
Objective To investigate the clinical characteristics and the related factors of peripapillary subretinal hemorrhage (PPSRH). Methods The clinical documents of fundus fluorescein angiography (FFA) of 23 patients (23 eyes) with PPSRH were retrospectively analyzed. Results All of the 23 eyes was myopes with middle or slight degree, and the corrected visual acuity was≥1.0. Among the 23 patients, 9 eyes were PPSRH, 13 eyes were PPSRH with disc hemorrhage, and 1 eye was PPSRH with disc and vitreous hemorrhage. All of the PPSRH was localed at the nasal edge of optic disc. Through FFA the hemorrhage showed blocked fluorescence and the optic disc showed nodular hyperfluorescence at the late phase, and nothing abnormal in the unaffected eyes. Conclusion PPSRH might be related to buried optic disc drusen. (Chin J Ocul Fundus Dis, 2002, 18: 96-97)
Since November 1974 to December 1993, 110 cases with malignant tumors of the extremities were treated by en bloc resection and limb salvage procedure in our hopsital. There were 57 males and 53 females. Their ages ranged from 14 to 70 years. The diagnosis of all patients were confirmed by pathology. Among them, 68 cases were malignant bone tumor, 42 cases were malignant tumor of soft tissue. If the soft tissue was involved by the malignant tumors, the tumor was resected radically and myocutaneous or skin flap was transferred to covered the defect. Among the 42 cases, 5 were free vascularized and 37 were pediculated flaps. The maximal size of the flap was 15×25 cm and the minimal was 6×8 cm. For the malignant bone tumors, en bloc resection was performed and the bone defect was reconstructed with autogenous free vascularized fibular graft, autograft of massive bone with fusion or allograft of cryogenic massive bone. If the joint was involved, limited excision with replacement of prosthesis was recommended. When both soft tissue and bone were involved, segmental resection of tumor was indicated in 17 cases. The patients were followed up for 2 to 9 years, the results of 53 cases were still alive with a survival rate of 48.2%. We emphasized that the local extensive resection should be performed with a safety margin of 3 to 5 cm beyond the tumor. A djuvant chemotheapy and radiotherapy had definite value in the treatment of some malignant tumors, so that preoperative or postoperative chemotherapy or radiotherapy was necessary. Sometimes radical resection of regional lymph rodes was necessary. The indications and causes of recurrence were discussed.
Objective To investigate the damage to the retinal cells and apoptosis of retinal cells of rats after ischemia-reperfusion insult. Methods The retinal ischemia-reperfusion model was developed by increasing intraocular pressure to 109725 mm Hg in rat eyes. Morphological changes of the rat eyes were observed by means of routine histopathology with HE staining. Apoptosis of the retina was assayed by both DNA fragmentation gel-electrophoresis and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick end labelling (TUNEL). Results Compared with the normal control, no histopathological changes were revealed in the rat retinas 30 min after the ischemia and then reperfued for 24 h or 48 h. Retinal ganglion cell layer (RGL) and inner plaxiform layer (IPL) of the retina were observed, however, to become significantly thinner 60 min after the ischemia and then reperfued for 24 h or 48 h. Together with the pathological changes DNA ladder pattern was detected in the same group of the rats. Further, immunochemical stain of the eye demonstrated that TUNEL positive cells were localized in RGL and IPL of the retina. Conclusion Ischemia-reperfusion insult of the eye may remarkably damage the retina of the rat eye. The damage to the retinal cells is mainly localized within RGL and IPL and apoptosis is the important mechanism of the retinal disorder. (Chin J Ocul Fundus Dis, 2002, 18: 296-298)
目的:研究早期连续性静脉-静脉血液滤过和反复间断静脉一静脉血滤治疗重症急性胰腺炎的临床价值。 方法:45例SAP患者随机分为CVVH组(25例)和RIVVH组(20例)。比较2组患者的生命体征、生化指标、治疗前后的APACHE Ⅱ评分、死亡率、好转率。 结果:CVVH 组中3例死于MODS,死亡率为12％,RIVVH组死亡3例 (15％),其差异有统计学意义(Plt;0.05);CVVH 组好转率为80％ (20/25),与RIVVH组的80％(16/20)一致。在治疗的早期阶段,对于改善生命体征,降低APACHEⅡ评分CVVH效果更显注,但随着治疗时间的延长,这种差异逐渐变小,在后期两组间不再存在疗效差异,最终疗效上没有明显的差异,而RIVVH组,在治疗费用和人员成本上具有优势。结论:早期RIVVH 治疗SAP可以作为SAP重要的辅助治疗措施。
Objective To describe cultured human retinal pigment epithelial (RPE) cells transdifferentiation and investigate the effects of human vitreous fluid on the morphologic and cytoskeleton changes of RPE cells in vitro. Methods Cytoskeleton characteristics in the 2nd, 5th, 8th passage of RPE cells in normal culture, which included cytokeratin 18 (CK18) and α-smooth muscle actin (α-SMA) were analyzed by Western blot. RPE cells were cultured in human vitreous-conditioned medium (VCM) at the concentration of 1∶4 for 6 days, morphologic changes were examined by light and electron microscopy, and cytoskeleton characteristics were analyzed by imunocytochemistry and Western blot. Results During culture in vitro, RPE cells lost epithelial characteristics and aquired fibroblast-like phenotype. The expression of CK18 was the highest at the 5th passage, and it decreased in the following passage, but α-SMA increased gradually. The morphologic transdifferentiation from epithelial to fibroblast-like cells of RPE was accelerated by VCM. Ultrastructural changes such as decreased microvilli and gradually increased rough endoplasmic reticulum and Golgi complex were found during the cultivation. CK18 produced by RPE cells decreased in VMC (P＜0.05), and α-SMA increased (P＜0.01). Conclusion Morphologic changes in epithelialmesenchymal transdifferenetiation of RPE cells are stimulated by VCM and accomplied by the shift of cytoskeleton proteins, The results imply that cells migration may be decreased and contraction may be enhanced in VCM. It may suggest that vitreous accelerates the pathogenesis of PVR and RPE cells play an important role. (Chin J Ocul Fundus Dis, 2002, 18: 289-292)
Objective To detect the difference of the light sensitivity in the central visual field between normal people and type Ⅱ diabetic patients without diabetic retinopathy, and evaluate the effect of perimetric examination in early diagnosis of diabetic retinopathy. Methods The light sensitivity at the 80 locations in the central field was measured by Dicon field analyzer (model TKS-4000) in 76 normal eyes of 44 normal volunteers aged from 45 to 72 years and 75 eyes of 40 type Ⅱ diabetic patients without retinopathy aged from 46 to 71 years. Results For the diabetic patients without diabetic retinopathy, the light sensitivity of locations decreased by 4-8 dB,and there were some decreased light sensitivity areas. The mean light sensitivity of three zones of the central field had significant reduction in the diabetic patients as compared with the control group(Plt;0.001). Conclusion The retinal neurosensory function of diabetic patients is damaged in some degrees before diabetic retinopathy occured, and no relationship is found between the decrease of retinal light sensitivity and localized blood-retinal barrier leakage. It is suggested that examination of central field with computerized perimetry has certain clinical significance in early diagnosis of diabetic retinopathy. (Chin J Ocul Fundus Dis, 2002, 18: 218-220)