Major Isolates Distribution and the Drug Resistance Changes of Multidrug-resistant Organism in 2013_West China Medical Journal

Authors：

 WangHuiling , LuXianlei

Department of Nosocomial Infection, the Fifth People's Hospital of Chengdu, Chengdu, Sichuan 611130, P. R. China;

Corresponding author：

Keywords：

Nosocomial infection; Drug resistance surveillance; Antibiotics

DOI：

10.7507/1002-0179.20160077

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To find out the major isolates distribution, drug resistance changes of multidrug-resistant organisms (MDRO) in 2013 for rational use of antibiotics and hospital infection control. Methods A total of 32 566 cultured bacteria samples from the patients diagnosed between January 1st and December 31st 2013 were collected, using conventional tube biochemical assays and semi-automatic automicrobic (AMS) for bacteria identification; and antimicrobial susceptibility testing, major drug resistance mechanism detection were performed according to CLSI documents. MDRO definition was made according to the 2011 international consensus from European Center for Disease Control and Prevention (CDC), American CDC, Clinical and Laboratory Standards Institute (CLSI), and Food and Drug Administration. The data was analyzed by WHOnet 5.6 software. Results We got 3 684 strains isolates, G^- accounted for 76.08%, G⁺ 16.80%, fungi 7.11%, and fastidious bacteria 17.29%. The top ten isolates in order were:E.coli, A.baumannii, Ps.aeruginosa, H.influenzae, K.pneumoniae, S.aureus, S.pneumoniae, A.fumigatus, M.catarrhalis and C.freundii. From the first quarter 2012 to the fourth quarter 2013, the extended spectrum β lactamases (ESBL)-producing E.coli increased from 40.23% to 53.54%, ESBL-producing K.pneumonia increased from 14.28% to 34.78%, XDR-A.baumannii increased from 62.38% to 99.25%, metalloenzyme-producing Ps.aeruginosa increased from 7.37% to 25.37%, methicillin resistant staphylococcus aureus increased from 23.81% to 58.70%, and VRE increased from 0.00% to 28.12%. Conclusion In the isolates, the percentage of G^- was the highest, and the rate of MDRO are all unremittingly raising, which suggests us should pay more attention to microbiology analysis, rational use of antibiotics, strengthening hospital infection control, reducing the bacterial resistance, and strengthening MDRO surveillance.

Citation： WangHuiling, LuXianlei. Major Isolates Distribution and the Drug Resistance Changes of Multidrug-resistant Organism in 2013. West China Medical Journal, 2016, 31(2): 287-292. doi: 10.7507/1002-0179.20160077 Copy

1.	李耘, 吕媛, 薛峰, 等. 卫生部全国细菌耐药监测网(Mohnarin) 2011-2012年革兰阳性菌耐药监测报告[J]. 中国临床药理学杂志, 2014, 30(3):251-259.
2.	胡付品, 朱德妹, 汪复, 等. 2013年中国CHINET细菌耐药性监测[J]. 中国感染与化疗杂志, 2014, 14(5):365-374.
3.	Versalovic J, Carroll KC, Pfaller MA, et al. Manual of clinical microbiology[M]. 10th ed. Washington, D.C:American Society for Microbiology, 2011.
4.	陈东科, 孙长贵. 实用临床微生物检验与图谱[M]. 北京:人民卫生出版社, 2011.
5.	王端礼, 李若瑜, 王爱平. 医学真菌学——实验室检验指南[M]. 北京:人民卫生出版社, 2005.
6.	中华检验医学杂志CLSI临床检验标准编译小组. 抗菌药物敏感性试验执行标准;第二十三版资料增刊[J]. 中华检验医学杂志, 2013, 33(1):M100-S23.
7.	李春辉, 吴安华. 医疗机构耐药菌MDR、XDR、PDR的国际标准化定义专家建议(草案)[J]. 中国感染控制杂志, 2011, 10(3):238-240.
8.	李多, 李光荣, 向成玉. 2008年临床分离病原菌分布及耐药性分析[J]. 中华医院感染学杂志, 2010, 20(8):1155-1158.
9.	林昌锋, 邢贻雄, 陈如寿. 综合性医院病原菌分布及耐药性分析[J]. 中华医院感染学杂志, 2009, 19(3):335-337.
10.	郭庆芳, 杨春艳. 我院2007-2008年细菌耐药性监测与分析[J]. 中国医院药学杂志, 2010, 30(16):1407-1410.
11.	李玉瑭, 于超, 杜亭亭, 等. 2010年医院临床分离细菌的耐药性监测[J]. 中华医院感染学杂志, 2012, 22(19):4379-4381.
12.	Simner PJ, Zhanel GG, Pitout J, et al. Prevalence and characterization of extended-spectrum β-lactamase- and AmpC β-lactamase-producing Escherichia coli:results of the CANWARD 2007-2009 study[J]. Diagn Microbiol Infect Dis, 2011, 69(3):326-334.
13.	张立平, 邓丽, 郑丽华, 等. 2007-2009年临床病原菌分布及耐药趋势分析[J]. 中华医院感染学杂志, 2011, 21(6):1220-1223.
14.	王卫华, 陈洁, 李春仙, 等. 泛耐药鲍氏不动杆菌β-内酰胺酶、膜孔蛋白、外排泵编码基因研究[J]. 中华医院感染学杂志, 2012, 22(10):2001-2004.
15.	Jilu Shen YP, Fang YP. Role of the outer membrane protein OprD2 in carbapenem-resistance mechanisms of Pseudomonas aeruginosa[J]. PLoS One, 2015, 10(10):e0139995.

1. 李耘, 吕媛, 薛峰, 等. 卫生部全国细菌耐药监测网(Mohnarin) 2011-2012年革兰阳性菌耐药监测报告[J]. 中国临床药理学杂志, 2014, 30(3):251-259.
2. 胡付品, 朱德妹, 汪复, 等. 2013年中国CHINET细菌耐药性监测[J]. 中国感染与化疗杂志, 2014, 14(5):365-374.
3. Versalovic J, Carroll KC, Pfaller MA, et al. Manual of clinical microbiology[M]. 10th ed. Washington, D.C:American Society for Microbiology, 2011.
4. 陈东科, 孙长贵. 实用临床微生物检验与图谱[M]. 北京:人民卫生出版社, 2011.
5. 王端礼, 李若瑜, 王爱平. 医学真菌学——实验室检验指南[M]. 北京:人民卫生出版社, 2005.
6. 中华检验医学杂志CLSI临床检验标准编译小组. 抗菌药物敏感性试验执行标准;第二十三版资料增刊[J]. 中华检验医学杂志, 2013, 33(1):M100-S23.
7. 李春辉, 吴安华. 医疗机构耐药菌MDR、XDR、PDR的国际标准化定义专家建议(草案)[J]. 中国感染控制杂志, 2011, 10(3):238-240.
8. 李多, 李光荣, 向成玉. 2008年临床分离病原菌分布及耐药性分析[J]. 中华医院感染学杂志, 2010, 20(8):1155-1158.
9. 林昌锋, 邢贻雄, 陈如寿. 综合性医院病原菌分布及耐药性分析[J]. 中华医院感染学杂志, 2009, 19(3):335-337.
10. 郭庆芳, 杨春艳. 我院2007-2008年细菌耐药性监测与分析[J]. 中国医院药学杂志, 2010, 30(16):1407-1410.
11. 李玉瑭, 于超, 杜亭亭, 等. 2010年医院临床分离细菌的耐药性监测[J]. 中华医院感染学杂志, 2012, 22(19):4379-4381.
12. Simner PJ, Zhanel GG, Pitout J, et al. Prevalence and characterization of extended-spectrum β-lactamase- and AmpC β-lactamase-producing Escherichia coli:results of the CANWARD 2007-2009 study[J]. Diagn Microbiol Infect Dis, 2011, 69(3):326-334.
13. 张立平, 邓丽, 郑丽华, 等. 2007-2009年临床病原菌分布及耐药趋势分析[J]. 中华医院感染学杂志, 2011, 21(6):1220-1223.
14. 王卫华, 陈洁, 李春仙, 等. 泛耐药鲍氏不动杆菌β-内酰胺酶、膜孔蛋白、外排泵编码基因研究[J]. 中华医院感染学杂志, 2012, 22(10):2001-2004.
15. Jilu Shen YP, Fang YP. Role of the outer membrane protein OprD2 in carbapenem-resistance mechanisms of Pseudomonas aeruginosa[J]. PLoS One, 2015, 10(10):e0139995.

Previous Article
Effects of Pilose Antler Blood Wine on Bone Mineral Density and T-lymphocyte Subsets in Ovariectomized Female Rats
Next Article
Study on the CT Features of Coronary Artery Aneurysm

West China Medical Journal

Major Isolates Distribution and the Drug Resistance Changes of Multidrug-resistant Organism in 2013

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content