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find Keyword "内皮素" 32 results
  • STUDYONMODIFIEDCOLDSTORAGEMETHODOFRATLIVERSWITHSELFMADEHYDSOLUTION

    To investigate the effect of modified cold preservation on rat livers. The modified method was that the vascular bed of rat liver was infused with an additional 20 ml, 30 ml and 40 ml self-made HYD solution/100g liver. Via portal vein and the vesselses of liver were ligated. Forty rats were randomly divided into four groups including conventional storage group (control group) and modified preservation groups (20%, 30% and 40% group). The indices of hepatic microcirculation, enzymes level in the effluent and bile production were determined in the groups. Results: Bile production and all the indices of hepatic microcirculation including portal perfused pressure, endothelin-1 in the effluent, Trypan Blue distrubution time and histology in experimental groups were significantly superior to those in control group (P<0.05). The liver enzymes in 30% group were markedly lower than those in control group (P<0.05). The preservation effect of rat livers in 30% group was the best among the experimental groups. Conclusions: The modified cold storage method is effective and may have potential for clinical application for liver preservation.

    Release date:2016-08-29 09:20 Export PDF Favorites Scan
  • 视网膜静脉阻塞血浆内皮素水平研究

    Release date:2016-09-02 06:11 Export PDF Favorites Scan
  • The Mechanisms of Endothelin-1 in Rat Paraquat Poisoning

    目的:观察百草枯中毒大鼠血浆和肺组织匀浆中的内皮素-1的动态变化,探讨大鼠PQ中毒肺损伤的可能机制。方法:将雄性Wistar大鼠随机分成百草枯中毒组和生理盐水对照组,分别给予16mg/kg百草枯或等体积生理盐水腹腔内注射,并在给药后6、12、24和72 h处死大鼠,观察其一般表现、肺的病理变化。测定肺系数、肺组织匀浆和血浆中内皮素-1的含量。结果: 百草枯中毒后大鼠出现呼吸困难,活动能力下降,肺系数增加,并随时间变化,光镜下表现为肺出血、水肿等不同程度的肺损伤改变。血浆和组织匀浆中内皮素-1在中毒后与对照组大鼠比较明显升高,并随时间变化(Plt;0.01)。结论:在百草枯中毒肺损伤中,内皮素-1可能发挥了重要作用。

    Release date:2016-09-08 10:04 Export PDF Favorites Scan
  • Protective Effect of Shuangdan Capsule on Myocardial Ischemia/Reperfusion Injury in Rats

    【摘要】目的探讨丹酚酸(Sal)B 配伍丹皮酚对大鼠心肌缺血再灌注损伤(MIRI)的保护作用。方法大鼠心肌缺血60 min 后再灌注90 min 造成大鼠心肌缺血再灌注损伤模型,测定给药后心肌组织匀浆、血浆中内皮素(ET)、血清中一氧化氮(NO)和一氧化氮合酶(NOS)的变化,并通过病理组织学检查,观察Sal B 配伍丹皮酚5、10、15 mg/kg对大鼠MIRI不同浓度和不同给药方法的治疗作用。结果Sa1 B配伍丹皮酚,中、高剂量组均能减少心肌组织和血浆中ET 的含量,提高NOS 的活性,增加NO的释放(Plt;005)。结论Sal B减轻大鼠MIRI 可能是通过调节ET/NO 系统的平衡,维持冠脉血管张力,改善心肌能量代谢障碍实现的。

    Release date:2016-09-08 09:31 Export PDF Favorites Scan
  • Achievements of Basic Research in Small-for-Size Liver Transplantation

    Objective To introduce the mechanisms of graft injuries after small-for-size liver transplantation and protective measures. Methods Recently relevant literatures were reviewed and summarized. Results Portal hypertension after small-for-size liver transplantation induces mechanical injuries as well as hepatic sinusoidal microcirculation disturbance and cytokines release, which worsened the injuries. Decrease portal pressure by surgery or drug could improve grafts function. ConclusionComprehending the mechanisms of graft injuries will contribute a lot for the living donor liver transplantation.

    Release date:2016-09-08 10:54 Export PDF Favorites Scan
  • Relationship between Inducible Nitric Oxide Synthase and Endothelin mRNA Expression and Intestinal Damage and Effect of Salvia in Acute Pancreatitis

    【摘要】目的探讨重症急性胰腺炎(SAP)时胰腺组织的诱导型一氧化氮合成酶(iNOS)、内皮素(ET1) mRNA表达状态, 以及与血浆中NO、ET1浓度和肠道损伤的关系及丹参治疗的影响。方法Wistar大鼠45只随机分为3组:SAP模型组(A组),SAP丹参治疗组(B组),假手术 组(C组),进行不同治疗和观察分析。结果A组血中淀粉酶(AML)、ET1、NO、内毒素(LPS)含量、125 I白蛋白累积指数及腹水量均显著高于C组(Plt;0.01);与A组比较,B组胰腺ET1和iNOS mRNA表达较弱,血中AML、ET1、NO、LPS及腹水量显著下降(Plt;0.01),125 I白蛋白累积指数较A组也有下降,但无差异(Pgt;0.05)。结论SAP时存在肠道损伤,胰腺组织ET1、iNOS mRNA的过度表达,使血中ET1、NO浓度升高,造成肠道屏障功能受损,肠通透性增加,引起内毒素血症。丹参注射液通过减轻SAP时胰腺的病理损害程度,下调胰腺ET1和iNOS mRNA的表达,使血中ET1、NO浓度下降,对SAP及其肠道损伤有一定治疗作用。

    Release date:2016-09-08 09:31 Export PDF Favorites Scan
  • STUDY ON THE CHANGE OF PLASMA ENDOTHELIN,LPS,TNF-α,IL-6 AND IL-8 IN PATIENTS WITH OBSTRUCTIVE JAUNDICE AFTER SURGERY

    Objective To research the changes in plasma endothelin, LPS, TNF-α, IL-6 and IL-8 in the patients with obstructive jaundice (OJ) and non-obstructive jaundice (NOJ) after surgery. Methods The plasma ET, LPS, TNF-α, IL-6 and IL-8 were measured in 15 patients without jaundice as controls. Results As compared with NOJ, the postoperative changes of ET, LPS, TNF-α, IL-6 and IL-8 in OJ group increased significantly at multiple time points (P<0.05). Conclusion There is interaction between ET,LPS, TNF-α, IL-6 and IL-8 and damage to multiple organ function in patients with OJ after surgery.

    Release date:2016-08-28 05:30 Export PDF Favorites Scan
  • 内皮素受体拮抗剂在围术期治疗6个月以下婴儿肺动脉高压的临床意义

    目的 评价非选择性内皮素受体拮抗剂(波生坦)治疗6个月以下婴儿围术期先天性心脏病肺动脉高压的临床效果。 方法 按世界卫生组织(WHO)肺动脉高压分级(FC)将60例年龄小于6个月的先天性心脏病(分流)相关性肺动脉高压患者分为两组,对照组:30例围术期接受常规治疗[包括:地高辛口服0.01 mg/(kg·d),1次/d;安体舒通口服3 mg/(kg·d),3次/d;卡托普利口服1.5 mg/(kg·d),3/d; 治疗组:30例在常规治疗基础上加用波生坦(术前6.25 mg,2次/d,1周;术后12.5 mg,2次/d,应用1~4周)口服治疗。观察两组患者肺动脉收缩压、运动耐量和机械辅助通气时间的变化,评估药物的临床效果。结果 治疗组用药后1周肺动脉收缩压较对照组明显改善63.43±7.37 mm Hg vs. 69.11±6.35 mm Hg,t=3.197,P=0.002),术后1周治疗组肺动脉压较对照组持续下降(42.42±8.13 mm Hg vs. 58.93±7.79 mm Hg,t= 8.030,P=0.001);治疗组术后机械辅助通气时间和运动耐量(30 ml奶液喂养时间)均明显优于对照组。 结论 波生坦用于治疗6个月以下的小婴儿是安全的,先天性心脏病(分流)相关的肺动脉高压患者围术期使用波生坦能显著降低肺动脉压,改善心功能及运动耐量,缩短术后机械辅助通气时间。

    Release date:2016-08-30 05:57 Export PDF Favorites Scan
  • Effect of Endothelin and Nitric Oxide Changes in Local Site of Vein Transfer with Delayed Breaking Pedicle in Rabbits

    Objective To study the changes of endothelin (ET) and nitric oxide (NO) in the local site of vein transfer with delayed breaking pedicle and the relation with vasospasm and vein transfer in rabbits. MethodsThe ET concentration of blood was determined with the radioimmunoassay method. The plasma NO-2,NO-3 levels in the local site of vein transfer with delayed breaking pedicle, which reflected NO levels indirectly, were detected with Ultravioletvisible (UvVIS ) spectrophotometer. ResultsThe endothelin concentration of blood was increased significantly at 2, 4 hour after the operation (P<0.01), and at 8 hour after the operation (P<0.05). The plasma NO level was significantly decreased at 2, 4 hour after the operation (P<0.01). But at 24 hour after the operation, the plasma NO level was increased significantly (P<0.05). Conclusion The recovery of ET concentration of blood and the increase of plasma NO at 24 hour after the operation are the cause of the reduced incidence of vascular crisis of vein transfer with delayed breaking pedicle, and the very time point is the optimum moment for pedicle breaking.

    Release date:2016-08-28 05:11 Export PDF Favorites Scan
  • EFFECT OF ENDOTHELIN-1 ON HEPATIC BLOOD FLOW IN RATS

    In order to study effect of endothelin (ET-1) on hepatic blood flow in rats and effect of nitric oxide (NO) and prostacyclin (PGI2) on ET-1 biological function, 20 rats were randomized into control, ET-1, ET-1 plus nitric-Larginine, ET-1 plus prostacyclin and indomethen groups. The result showed that ET-1 decreased hepatic blood flow and lasted for longer time. NO and PGI2 may antagonize the biological action of ET-1 during endotoxemia. Therefore, the endothelium-derived vascular factors may regulate hepatic blood flow.

    Release date:2016-08-29 03:18 Export PDF Favorites Scan
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