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Effects of Soybean Isoflavones on Antioxidative Action in Aged Rats

目的:研究大豆异黄酮对D半乳糖致衰老大鼠抗氧化能力的影响。方法:用D半乳糖注射Wistar雄性大鼠5个月,建立衰老模型。对致衰老模型组、大豆异黄酮组肝脏、心脏和前列腺丙二醛(MDA)含量、超氧化物歧化酶(SOD)、谷胱甘肽过氧化酶(GSHPx)活性进行测定及比较。结果:低、中、高不同剂量大豆异黄酮灌喂组与模型组大鼠相比,各脏器MDA含量(μmol/L)(心脏:695±093,562±112,435±112比802±111;肝脏:815±085,647±120,515±112比935±135;前列腺:715±092,558±115,423±125比833±124)均有降低,差异有统计学意义(Plt;005),而SOD酶活性(nmol/L)(心脏:4732±308,5518±428,6120±368比3225±370;肝脏:18121±506,19015±706,19720±570比17213±512;前列腺:4156±301,4607±421,5015±335比3374±305)和GSHPx酶活性(nmol/L)(心脏:905±096,1111±245,1313±146比713±151;肝脏:902±105,1150±223,1362±192比698±160;前列腺:435±085,613±102,747±155比312±106)有升高,差异同样具有统计学意义(Plt;005);大豆异黄酮摄入量越高,MDA含量越低,而SOD、GSHPx酶活性越高。结论:摄入适量大豆异黄酮可有效增强大鼠机体抗氧化能力,从而延缓D半乳糖诱发的大鼠衰老。

Thioredoxin-1: A potential target for prevention of heart-related reactive oxygen species injury

Reactive oxygen species (ROS) play an important role in the pathogenesis of various cardiovascular diseases, by leading to cell apoptosis and thus causing organic injuries. Anti-ROS therapy is highly anticipated, but currently, there is still no appropriate prevention method. Studies have shown that thioredoxin (Trx), being a kind of significant endogenous antioxidant system, has excellent antioxidant capacity. Promotion of Trx can reduce key biomolecules to eliminate ROS or regulate many signaling pathways, thus resisting ROS injuries, which may be a new anti-ROS strategy. Therefore, we reviewed the research progress of Trx in cardiac antioxidant therapy to discuss its potential and possibility to be a target for prevention of heart-related ROS injury.

急诊高压氧对急性一氧化碳中毒治疗的临床疗效观察

摘要:目的:探讨高压氧对急性一氧化碳中毒治疗的临床意义。方法:对120例急性一氧化碳中毒患者除常规治疗外，均施行急诊高压氧治疗。结果:接受急诊高压氧治疗的急性一氧化碳中毒患者有效率达100%。结论:高压氧对急性一氧化碳中毒的治疗有显著疗效。

Effect of inducible nitric oxide synthase or cyclooxygenase-2 on retinal neovasc ularization in oxygeninduced retinopathy mouse model

Objective To investigate the effect of inducible nitric oxide synth ase (iNOS) or cyclooxygenase－2 (COX－2) on retinal neovascularization and its possible mechanism in oxygen－induced retinopathy （OIR） mouse model. Methods Retinal neovascularization was induced by oxygen with different concentration. The expression of iNOS, COX－2, matrix metalloproteinases 2 (MMP－2) and vascular end othelial growth factor (VEGF) in the retinae of experimental animals were analyzed by immunohistochemistry, realtime polymerase chain reaction and western blotting technologies. Results The inhibition of COX－2 or iNOS obviously attenuated retinal neovascularization and decreased the expression of VEGF and MMP－2. The iNOS inhibition decreased COX－2 expression, and vice versa. Conclusions COX－2 and iNOS may play a role in retinal neovascularization in OIR mouse model, which may act by regulating the expression of VEGF and MMP－2.

连续性肾脏替代治疗串联体外二氧化碳清除技术治疗呼吸衰竭合并急性呼吸窘迫综合征一例

The Incidence of Acute Mountain Sickness and its Treatment

短期进入高原从事高强度工作所致高原反应是值得探讨的问题,查阅文献,探讨其病因及发病机理、临床表现,总结国内外在诊断、预防及治疗方面的经验,探索一套可行、有效的预防及治疗措施,具有重要的临床意义。

Expressions and Significances of HIF-1α and BAK in Gastric Cancer

目的　探讨HIF-1α和BAK蛋白在胃癌中的表达情况，以及二者在胃癌中的相互关系及作用。方法　应用免疫组化SABC染色法检测80例胃癌组织和20例正常胃组织中的HIF-1α和BAK蛋白的表达情况。结果　胃癌中HIF-lα和BAK蛋白的表达阳性率分别为56.3%（45/80）和67.5%（54/80），而在胃正常组织中分别为5.0%（1/20）和20.0%（4/20），二者在胃癌中的表达显著高于胃正常组织，其差异有统计学意义（P＜0.05）。HIF-1α蛋白表达与胃癌组织的浸润范围、分化程度及淋巴结转移有关（P＜0.05），与临床分期、年龄及性别无关（P＞0.05）；BAK蛋白表达与胃癌浸润及分化程度相关（P＜0.05），与淋巴结转移、临床分期、年龄及性别无关（P＞0.05）。胃癌组织中HIF-1α与BAK蛋白的阳性表达之间呈正相关（列联系数r=0.056，P＜0.05）。结论　HIF-1α与BAK蛋白在胃癌的临床分期及浸润转移中存在关系，这对于研究胃癌的发生和发展，以及对于探索以二者为靶点的抗肿瘤治疗有重要意义。

高压氧治疗不依从性的原因分析

目的　探讨高压氧治疗时患者不依从性的原因，以积累经验，指导临床工作。 方法　2011年1月－2012年1月间采用自制调查问卷进行回访，总结、分析未遵医嘱进行高压氧治疗患者的不依从性的原因。 结果　由于客观原因，患者高压氧治疗依从性与多种主观因素有关，因缺乏高压氧知识、恐舱、治疗不便等综合因素为主要原因。 结论　加强高压氧知识的普及和宣教，加强人文关怀，提高患者的依从性。

EXPERIMENTAL STUDY ON THE ROLE OF LIPID PEROXIDES DURING LUNG INJURY INDUCED BY ACUTE CHOLANGITIS OF SEVERE TYPE

The contents of lipid peroxides(LPO)and vitamin E(V.E)and some functional index and histologic changes in the lungs from the the rabbit models of acute cholangitis of severe type(ACST)were measured dynamically.The results revealed that the V.E content decreased strikingly from 6 hours and the LPO level increased progressivelg from 12 hours in the lungs.Simultanuosly,the congestion and neutrophil infiltreation in the lung mesenchyme,and the endothelial cell damage and thrombosis in the lung blood capillaries had been observed.These suggest that acute lung injury induced by ACST is referable to the lipid peroxidation damage to the lung blood capillaries which is due to increased LPO and decreased antioxidants including V.E.

麻醉苏醒室患者低氧血症的护理

【摘要】　目的　分析麻醉苏醒室（postanesthesia care unit，PACU）患者发生低氧血症的原因，及纠正处置方法。　方法　对2010年2-10月全身麻醉手术后入PACU的12 485例患者资料进行回顾性分析，了解低氧血症的发生率及处理前后脉搏血氧饱和度（pulse oximetry，SpO2）、呼吸、心率和Ramsay评分的比较。　结果　本研究显示低氧血症的发生率0.94%，处理前后SpO2、心率和Ramsay评分差异有统计学意义。所有入PACU全身麻醉患者中无死亡。　结论　对入PACU的术后全身麻醉患者进行评估及严密观察，及时分析处理低氧血症，减少并发症的发生，保证患者在PACU期间的安全与恢复。